20.500.12556/RUL-169396 Discovery of novel biomarkers with extended non-coding RNA interactor networks from genetic and protein biomarkers Curated online interaction databases and gene ontology tools have streamlined the analysis of highly complex gene/protein networks. However, understanding of disease pathogenesis has gradually shifted from a protein-based core to complex interactive networks where non-coding RNA (ncRNA) is thought to play an essential role. As current gene ontology is based predominantly on protein-level information, there is a growing need to analyze networks with ncRNA. In this study, we propose a gene ontology workflow integrating ncRNA using the NPInter V5.0 database. To validate the proposed workflow, we analyzed our previously published curated biomarker datasets for hidden disease susceptibility processes and pharmacogenomics. Our results show a novel involvement of melanogenesis in psoriasis response to biological drugs in general. Hyperpigmentation has been previously observed in psoriasis following treatment with currently indicated biological drugs, thus calling attention to melanogenesis research as a response biomarker in psoriasis. Moreover, our proposed workflow highlights the need to critically evaluate computed ncRNA interactions within databases and a demand for gene ontology analysis of large miRNA blocks. disease pathogenesis gene ontology untranslated RNA biomarkers non-coding RNA true false true Angleški jezik Ni določen Članek v reviji 2025-05-27 13:14:58 2025-05-27 13:15:00 2025-05-28 04:11:59 0000-00-00 00:00:00 2024 0 0 16 str. iss. 18, art. 10210 Vol. 25 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 575 1422-0067 10.3390/ijms251810210 208743939 ijms-25-10210.pdf ijms-25-10210.pdf 1 312FD11058FC10FB3A828D916E2F0E4F b8e0e068a4a2aef6fa9fbb00d2ab4291da1c020f46b90d3e0e45ca077bf3dfad d9a5ac55-3aea-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210719 https://www.mdpi.com/1422-0067/25/18/10210 1 8a0cae22-3aea-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210718 Medicinska fakulteta 0 0 0