20.500.12556/RUL-169189 Načrtovanje in izvedba regulacije temperature v bioreaktorju Design and implementation of temperature control in a bioreactor Cilja diplomskega dela sta bila načrtovanje in izvedba temperaturne regulacije razvojnega bioreaktorja. Bioreaktor je ključna naprava v biotehnoloških procesih, ki omogoča nadzorovano gojenje različnih sevov bakterij. Bioreaktor v zavodu CO BIK se uporablja predvsem za gojenje bakterije Escherichia coli (E.coli). E.Coli se pogosto uporablja kot gostitelj v bioprocesih za produkcijo plazmidne DNA (pDNA), ki ima široko uporabnost v biomedicinskih in farmacevtskih aplikacijah. Proces vključuje optimizacijo rastnih pogojev bakterij, kot so temperatura, pH, razmerje raztopljenega kisika in sestava hranilnega medija. Za zagotovitev ustrezne temperature klasični manjši bioreaktorji uporabljajo termični plašč. Dokazal sem, da je ustrezna regulacija temperature možna tudi z grelnim in hladilnim prstom, ki nadomestita termični plašč v razvojnem bioreaktorju. S PI-reguliranjem temperature z grelnim in hladilnim prstom zagotavljamo pogoje, ki jih zahteva biološki proces gojenja bakterij. Postopek regulacije temperature v bioreaktorju sem najprej izvedel brez uporabe bakterij. Po uspešni PI-regulaciji grelnega in hladilnega prsta se je uspešno izvedlo gojenje bakterij. V času pisanja diplomskega dela sem projekt zaključil. Uporaba razvojnega bioreaktorja omogoča stabilno in ponovljivo proizvodnjo plazmidov, kar je ključno za nadaljnjo uporabo v sintetični biologiji, genskem inženiringu ter personalizirani medicini. Razvojni bioreaktor je trenutno v uporabi za razvoj in optimizacijo procesov proizvodnje pDNA. The aim of the bachelor's thesis was the design and implementation of temperature control for a developmental bioreactor. A bioreactor is a key device in biotechnological processes that enables the controlled cultivation of various bacterial strains. The bioreactor at CO BIK is mainly used for cultivating the bacterium Escherichia coli (E. coli). E. coli is frequently used as a host in bioprocesses for the production of plasmid DNA, which has wide applications in biomedical and pharmaceutical fields. The process involves the optimization of bacterial growth conditions, such as temperature, pH, dissolved oxygen concentration, and the composition of the growth medium. To ensure the appropriate temperature, traditional smaller bioreactors use a thermal jacket. I demonstrated that adequate temperature control is also possible using a heating and cooling finger, which replaces the thermal jacket in the developmental bioreactor. With PI temperature control using the heating and cooling finger, we provide the conditions required by the biological process of bacterial cultivation. I first carried out the temperature control procedure in the bioreactor without using bacteria. After the successful PI control of the heating and cooling finger, the cultivation of bacteria was successfully executed. During the writing of the thesis, I completed the project. The use of the developmental bioreactor enables stable and reproducible production of plasmids, which is crucial for further applications in synthetic biology, genetic engineering, and personalized medicine. The developmental bioreactor is currently used for the development and optimization of processes for the production of plasmid DNA (pDNA). bioreaktor regulacija temperature PID regulator bioreactor temperature control PID controller true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-05-16 11:50:12 2025-05-16 11:50:19 2025-06-19 13:17:21 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 62961 239972867 Malik_Dominik_-_Nacrtovanje_in_izvedba_regulacije_temperature_v_bioreaktorju.pdf Malik_Dominik_-_Nacrtovanje_in_izvedba_regulacije_temperature_v_bioreaktorju.pdf 1 72E53C0F113BB912D299B7050E378F6F e758d3e6101bb24ca7982ac0b48fcf5dcbca1595d30b51a6424313dabf501a01 f1e348c2-3239-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210399 Fakulteta za elektrotehniko 0 0 0