20.500.12556/RUL-169163 Testiranje stabilnosti hidrolatov prave sivke (Lavandula angustifolia) in rožmarina (Rosmarinus officinalis) Stability testing of lavender (Lavandula angustifolia) and rosemary (Rosmarinus officinalis) hydrolates V magistrski nalogi smo proučevali sestavo in stabilnost hidrolatov prave sivke (Lavandula angustifolia) in navadnega rožmarina (Rosmarinus officinalis, syn. Salvia rosmarinus) z uporabo metode plinske kromatografije, sklopljene z masno spektrometrijo (GC-MS). Iz sveže nabranih rastlin smo s postopkom destilacije pridobili eterična olja in hidrolate obeh rastlin. Hidrolate smo shranjevali pod različnimi pogoji pet mesecev, da bi spremljali njihovo stabilnost. Pogoji shranjevanja so vključevali: stekleno ovojnino v hladilniku, stekleno ovojnino pri 40 °C, stekleno ovojnino pri 20–25 °C (sobni temperaturi) v temi, stekleno ovojnino pri 20–25 °C, izpostavljeno svetlobi, ovojnino iz HDPE pri 20–25 °C v temi, kovinsko ovojnino pri 20–25 °C v temi in ovojnino iz PE pri 20–25 °C. V mesečnih intervalih smo tako shranjene hidrolate analizirali z GC-MS, spremljali spremembe v vsebnosti hlapnih spojin in določili koncentracije glavnih hlapnih spojin s pomočjo standardnih raztopin znanih koncentracij. Z analizo sestave smo potrdili povezavo med stabilnostjo hidrolatov in pogoji shranjevanja. Hidrolati, shranjeni v hladilniku, so skozi čas pokazali najmanjše spremembe v kemijski sestavi. Nasprotno pa so hidrolati, shranjeni pri povišani temperaturi (40 °C) ali izpostavljeni svetlobi, kazali izrazitejše spremembe v številu in koncentracijah ključnih hlapnih spojin. Tudi material ovojnine je vplival na stabilnost; steklene ovojnine so zagotavljale boljšo zaščito v primerjavi s plastično ali kovinsko ovojnino. Na podlagi rezultatov smo zaključili, da je za optimalno ohranitev kemijske sestave in kakovosti hidrolatov sivke in rožmarina priporočljivo shranjevanje v stekleni ovojnini v hladilniku ali vsaj v temi pri sobni temperaturi (20–25 °C). Te ugotovitve so pomembne za pravilno shranjevanje in določanje roka uporabnosti hidrolatov, ki jih uporabljamo v kozmetiki, aromaterapiji, prehrani in na drugih področjih. In this thesis, the composition and stability of the hydrolates of lavender (Lavandula angustifolia) and rosemary (Salvia rosmarinus) were studied using gas chromatography coupled to mass spectrometry (GC-MS). Essential oils and hydrolates were obtained from freshly harvested plants through the distillation process. The hydrolates were stored under different conditions for five months to monitor their stability. The storage conditions included: glass packaging in a refrigerator, glass packaging stored at 40 °C, glass packaging at 20–25 °C (room temperature) in the dark, glass packaging at 20–25 °C exposed to light, HDPE packaging at 20–25 °C in the dark, metal packaging at 20–25 °C in the dark, and PE packaging at 20–25 °C. At monthly intervals, the stored hydrolates were analyzed using GC-MS to track changes in the content of volatile compounds, and the concentrations of the main volatile compounds were determined using standard solutions of known concentrations. The composition analysis confirmed the relationship between hydrolate stability and storage conditions. Hydrolates stored in a refrigerator exhibited the least changes in chemical composition over time. In contrast, hydrolates stored at elevated temperatures (40 °C) or exposed to light showed more pronounced changes in the number and concentration of key volatile compounds. The packaging material also influenced stability; glass containers provided better protection compared to plastic or metal packaging. Based on the results, we concluded that to optimally preserve the chemical composition and quality of lavender and rosemary hydrolates, storage in glass containers in a refrigerator or at least in the dark at room temperature (20–25 °C) is recommended. These findings are essential for proper storage and determining the shelf life of hydrolates used in cosmetics, aromatherapy, food, and other fields. hidrolat GC-MS eterično olje prava sivka (Lavandula angustifolia) rožmarin (Rosmarinus officinalis syn. Salvia rosmarinus) hydrolat GC-MS essential oil true lavender (Lavandula angustifolia) rosemary (Rosmarinus officinalis syn. Salvia rosmarinus) true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-05-16 07:45:06 2025-05-16 07:45:14 2025-05-17 04:18:22 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 114747 26966.pdf 26966.pdf 1 4DAD9B6863769B8317E0F18C9E84C92A 3098aafe18e41e277f9efa37a24c12fbba9144d55756f0f07bbe1f1e6d409e18 b5125edf-3217-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210360 Fakulteta za farmacijo 0 0 0