20.500.12556/RUL-169105 Effective targeting of E2F1 transcription factor via siRNA gene electrotransfer in HT-29 colorectal carcinoma xenografts Colorectal cancer (CRC) remains a significant global health concern, with survival outcomes heavily dependent on the stage at diagnosis. Targeted therapies offer a promising approach to improve patient outcomes, particularly by addressing molecular drivers of tumor progression. One such target is the E2F1 transcription factor, a key regulator of the cell cycle and a contributor to proliferation, differentiation, apoptosis, metastasis, and chemoresistance in CRC. Previous studies have demonstrated the efficacy of E2F1 silencing via siRNA-loaded nanoliposomes in reducing tumor cell growth, but challenges such as immunogenicity and off-target effects have limited their in vivo application. In this study, we evaluated the potential of gene electrotransfer (GET) as a non-viral delivery system for delivery of therapeutic siRNA targeting E2F1 in the HT-29 CRC model. In vitro experiments showed effective silencing of E2F1 expression and a significant reduction in HT-29 cell survival. Subsequent in vivo studies confirmed the therapeutic potential of siE2F1 GET, with results demonstrating tumor growth delay, decreased proliferation, and increased necrosis in the tumors. This study establishes proof-of-principle for targeting E2F1 in CRC using GET, showcasing its versatility and therapeutic potential. colorectal carcinoma E2F1 gene electrotransfer GET silencing siRNA kolorektalni rak E2F1 GET elektroprenos genov true false true Angleški jezik Slovenski jezik Članek v reviji 2025-05-12 13:00:27 2025-05-12 13:00:32 2025-05-13 04:01:27 0000-00-00 00:00:00 2025 0 0 10 str. art. 108994 Vol. 165 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 1567-5394 10.1016/j.bioelechem.2025.108994 235435267 1-s2.0-S1567539425000970-main.pdf 1-s2.0-S1567539425000970-main.pdf 1 D3BF949517000A3A0EE3C24A8DD1A7E8 ed2d6bc8a5fc0b3f39ddedf268a376ece6dd92709d545935993285b8826ec716 5f9d7d7d-2f1f-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210267 https://www.sciencedirect.com/science/article/pii/S1567539425000970 0 58dc3d7a-2f1f-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210266 Medicinska fakulteta Biotehniška fakulteta 0 0 0