20.500.12556/RUL-169101 Interactive effects of high n-3 PUFA intake and cyclic heat stress under two dietary antioxidant levels in broiler chickens The study examined the combined effects of a diet high in n-3 PUFAs and cyclic heat stress (HS) considering two levels of dietary antioxidants. A total of 192 one-day-old male Ross 308 broilers were divided into eight groups in a 2 × 2 × 2 factorial design: thermoneutral (TN) or heat-stressed (34°C ± 1°C for 7 h/day from day 22) × fed a diet low in antioxidants (NRC group) or high in antioxidants (HAOX group; supplemented with a mixture of 200 IU/kg vitamin E, 250 mg/kg vitamin C and 0.15 mg/kg selenium) and supplemented or not with 5% linseed oil, forming the NRC N-3 and HAOX N-3 groups. High intake of n-3 PUFAs increased plasma F2-isoprostane and malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity. Cyclic HS decreased final body weight (BW) and average daily feed intake (ADFI) and increased DNA damage and serum corticosterone (CORT) levels. In addition, the changes in blood biochemistry indicated that the broilers were undergoing respiratory alkalosis. Interactions between n-3 PUFAs and HS were observed in liver MDA levels, plasma γ-tocopherol levels and serum alkaline phosphatase (AP) activity. Antioxidants increased blood levels of α-tocopherol, vitamin C, lipid- and water-soluble antioxidants and glutathione peroxidase (GPx) activity, while decreasing MDA, F2-isoprostane, CORT and AP levels. High intake of n-3 PUFA in combination with cyclic HS had negative effects on the health status of the broilers, which were alleviated by additional antioxidant supplementation. cyclic heat stress n-3 PUFA oxidative stress selenium vitamin C vitamin E perutnina pitovni piščanci prehrana živali vročinski stres maščobne kisline PUFA antioksidanti true false true Angleški jezik Slovenski jezik Članek v reviji 2025-05-12 11:13:51 2025-05-12 11:13:53 2025-05-13 04:01:26 0000-00-00 00:00:00 2025 0 0 15 str. art. 1594095 Vol. 16 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 636.5.084/.087 1664-042X 10.3389/fphys.2025.1594095 234703619 RAZ_Rezar_Vida_2025.pdf RAZ_Rezar_Vida_2025.pdf 1 8957D538E9F4EFA81A42E78F0E6D47C1 d29d1d882be27d4d60cc40fa03799fec2e3d68ca1c72dc873d06c235bb1aeba2 627580d2-2f12-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210260 https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2025.1594095/full 1 37ebc061-2f10-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210259 Biotehniška fakulteta Veterinarska fakulteta 0 0 0