20.500.12556/RUL-169072 Nontargeted urinary profiling strategy for endocrine-disrupting chemicals in women with ovarian malignancies Endocrine-disrupting chemicals (EDCs), including known and unknown parent compounds, their metabolites, and transformation products, are pervasive in daily life, posing increasing risks to human health and the environment. This study employed a high-resolution mass spectrometry-based nontargeted screening approach, integrating polar (HILIC) and reversed-phase separations to expand the chemical space coverage and, supported by open-science tools and resources, evaluated urinary chemical profiles to assess internal EDC exposure. Among 106 annotated biomarkers of exposure, six exhibited significantly higher normalized intensities in patients with ovarian malignancies compared to healthy controls (p < 0.05). This suggests their greater exposure to phthalates (diethylhexyl phthalate and diethyl phthalate), pesticides (metolachlor metabolite and 4-nitrophenol), a UV filter (benzophenone-1), and an industrial byproduct (4-methyl-2-nitrophenol). These compounds may interfere with hormonal regulation, potentially contributing to cancer development. While these findings highlight potential differences in internal EDC exposure, the study primarily demonstrates the applicability of nontargeted urinary profiling for chemical exposure assessment. By providing new insights into EDCs burden and its pathological implications, this work contributes to advancing next-generation chemical risk assessment within the European Partnership for the Assessment of Risks from Chemicals initiative and supports the development of preventive strategies to mitigate environmental cancer risks. ovarian cancer biomarkers exposure nontargeted screening high-resolution mass spectrometry analytical coverage reversed-phase HILIC anatomy cancer metabolism organic compounds solvents true false true Angleški jezik Ni določen Članek v reviji 2025-05-09 13:59:59 2025-05-09 14:00:08 2025-05-10 04:10:33 0000-00-00 00:00:00 2025 0 0 Str. 8380−8390 iss. 17 Vol. 59 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 61+502.1 1520-5851 10.1021/acs.est.4c13290 234015491 plesnik-et-al-2025-nontargeted-urinary-profiling-strategy-for-endocrine-disrupting-chemicals-in-women-with-ovarian.pdf plesnik-et-al-2025-nontargeted-urinary-profiling-strategy-for-endocrine-disrupting-chemicals-in-women-with-ovarian.pdf 1 B5AEF4F3D85BE48D80DDBCA0BC175D76 9afaaf337c310336958d1c52513c59dd622b41b67336e1662ebcdf46b6349a0a 29fb0cdd-2ccc-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210220 https://pubs.acs.org/doi/10.1021/acs.est.4c13290 1 f40f88f4-2ccb-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210219 Fakulteta za računalništvo in informatiko Medicinska fakulteta 0 0 0