20.500.12556/RUL-169064 Vitamin A-enriched diet increases urothelial cell proliferation by upregulating Itga3 and Areg after cyclophosphamide-induced injury in mice Vitamin A (VitA) is an essential nutrient, affecting many cell functions, such as proliferation, apoptosis, and differentiation, all of which are important for the regeneration of various tissues. In this study, we investigated the effects of a VitA-enriched diet on the regeneration of the urothelium of the urinary bladder in mice after cyclophosphamide (CP)-induced injury. Female mice were fed VitA-enriched and normal diet for 1 week before receiving an intraperitoneal injection of CP (150 mg/kg). Urinary bladders were removed 1 and 3 days after CP. On Day 1, RNA sequencing showed that VitA upregulated two Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathways: the cell cycle and the PI3K-Akt pathway. This was confirmed by qPCR, which showed significantly increased expression of the Itga3 and Areg genes. In addition, the effect of VitA on the proliferation of urothelial cells was analyzed by immunohistochemistry of Ki-67, which confirmed an increased proliferation rate. No significant effects of the VitA-enriched diet were observed on the expression of apoptosis-related genes and on differentiation-related markers of superficial urothelial cells. Our results suggest that a VitA-enriched diet improves early urothelial regeneration after CP-induced injury by promoting cell proliferation. apoptosis cyclophosphamide differentiation injury proliferation urinary bladder urothelium vitamin A apoptoza ciklofosfamid diferenciacija poškodba širjenje sečni mehur urotelija vitamin A true false true Angleški jezik Slovenski jezik Članek v reviji 2025-05-09 10:52:37 2025-05-09 10:52:40 2025-05-10 04:10:33 0000-00-00 00:00:00 2025 0 0 14 str. iss. 9, art. e70045 Vol. 69 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006:576 1613-4133 10.1002/mnfr.70045 232348419 Molecular_Nutrition_Food_Res_-_2025_-_Dragar_-_Vitamin_A‐Enriched_Diet_Increases_Urothelial_Cell_Proliferation_by.pdf Molecular_Nutrition_Food_Res_-_2025_-_Dragar_-_Vitamin_A‐Enriched_Diet_Increases_Urothelial_Cell_Proliferation_by.pdf 1 58FDB08F13552FB10E891C5F5E40931F da28dab44026045afe16be79cffd9b433aa65d6d833c9c98db3750bea5099698 1260c392-2cb2-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210205 https://onlinelibrary.wiley.com/doi/10.1002/mnfr.70045 1 c3eca080-2cb1-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210203 Medicinska fakulteta 0 0 0