20.500.12556/RUL-168620 Genome-wide identification and expression profiling of glycosidases, lipases, and proteases from invasive asian palm weevil, Rhynchophorus ferrugineus The red palm weevil, Rhynchophorus ferrugineus, is a destructive, invasive pest to a diverse range of palm plantations globally. Commonly used broad-range chemical insecticides for insect control pose high risks to non-target organisms, humans, and the environment. A bio-rational approach of screening natural small-molecule inhibitors that specifically target R. ferrugineus proteins critical to its life processes can pave the way for developing novel bioinsecticides. Digestive enzymes (DEs), which impair feeding on plants (herbivory), are promising targets. We generated de novo transcriptomes, annotated DE-related genes from the R. ferrugineus gut and abdomen, manually annotated the DE gene family from the recently available genome and our transcriptome data, and reported 34 glycosidases, 85 lipases, and 201 proteases. We identified several tandem duplicates and allelic variants from the lipase and protease families, notably, 10 RferLip and 21 RferPro alleles, which emerged primarily through indels and single-site substitution. These alleles may confer enhanced digestive lipolysis and proteolysis. Phylogenetic analyses identified and classified different subfamilies of DEs and revealed close evolutionary relationships with other coleopterans. We assessed select candidate DEs’ activity and the potential for inhibition in silico to better understand the herbivory arsenal. In silico analysis revealed that the selected enzymes exhibited similar ligand-binding affinity to their corresponding substrate, except for protease aminopeptidase N, RferPro40, which exhibited poorer affinity to the inhibitor bestatin. Overall, our study serves as a foundation for further functional analysis and offers a novel target for the development of a novel bio-rational insecticide for R. ferrugineus. red palm weevil Rhynchophorus ferrugineus herbivory digestive enzyme gene duplication phylogeny molecular docking palmov rilčkar Rhynchophorus ferrugineus škodljivci rastlin prebavni encimi podvajanje genov filogenija molekularna genetika true false true Angleški jezik Slovenski jezik Članek v reviji 2025-04-18 13:44:16 2025-04-18 13:44:23 2025-04-19 04:37:04 0000-00-00 00:00:00 2025 0 0 27 str. iss. 4, art. 421 Vol. 16 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 60 2075-4450 10.3390/insects16040421 233303043 https://www.mdpi.com/2075-4450/16/4/421 1 587e83bc-1c49-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=209506 Genome-Wide_Identification_and_Expression_Profiling_of_Glycosidases,_Lipases,_and_Proteases_from_Invasive_Asian_Palm_Weevil,_Rhynchophorus_ferrugineus.pdf Genome-Wide_Identification_and_Expression_Profiling_of_Glycosidases,_Lipases,_and_Proteases_from_Invasive_Asian_Palm_Weevil,_Rhynchophorus_ferrugineus.pdf 1 D15519A0A8B6EB340D60A2ACFA49C175 34a2e99452aabc2a9ae9f075fb09825e2d3e33eaf707a683402e6659894ebfce 8507a7e3-1c49-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=209507 Biotehniška fakulteta 0 0 0