20.500.12556/RUL-168269 Biofilm removal from difficult-to-reach places via secondary cavitation within a constrained geometry mimicking a periodontal/peri-implant pocket Biofilm removal from the apical region of the periodontal or peri-implant pocket, which is very difficult to achieve with mechanical instruments, is a major unresolved issue in dentistry. Here, we propose the use of photoacoustically induced streaming and secondary cavitation to achieve superior cleaning efficacy in the apical region of the periodontal and peri-implant pocket. We have used a prefabricated narrow wedge system that mimics the consistency of periodontal and peri-implant pockets of both healthy and severely inflamed tissue. We studied the effect of single-pulse modality Er:YAG on Pseudomonas aeruginosa biofilm removal. We used different laser energies, fiber-tip positions, and laser treatment durations. The cleaning process was monitored in real-time with a high-speed camera after each individual laser pulse application. The obtained results suggest that biofilm cleaning efficacy in a difficult-to-reach place in healthy model tissue is directly related to the onset of secondary cavitation bubble formation, which correlates with a significant improvement of biofilm removal from the apical region of the periodontal or peri-implant pocket. In comparison to the healthy tissue model, the laser energy in inflamed tissue model had to be increased to obtain comparable biofilm cleaning efficacy. The advantage of photoacoustic cavitation compared to other methods is that laser-induced cavitation can trigger secondary cavitation at large distances from the point of laser application, which in principle allows biofilm removal at distant locations not reachable with a laser fiber tip or other mechanical instruments. biofilm removal periodontal pocket peri-implant pocket cavitation photoacoustic cleaning Er:YAG USP biofilm zobni vsadki obzobni žepek fotoakustično odstranjevanje biofilmov lasersko čiščenje kavitacija true false true Angleški jezik Slovenski jezik Članek v reviji 2025-04-07 12:08:01 2025-04-07 12:08:06 2025-04-08 04:02:34 0000-00-00 00:00:00 2024 0 0 8 str. art. 106832 Vol. 104 Mar. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.61:544.537 1350-4177 10.1016/j.ultsonch.2024.106832 186706179 Volk,_Savli_..._Biofilm.pdf Volk,_Savli_..._Biofilm.pdf 1 C41A9E25876ECBE70596A21052AFA4CA d34475ebc7fb66f824a16c625a1f3d058fd92a374b501922e6d639d60d5d810d c44100ab-1397-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=201414 https://www.sciencedirect.com/science/article/pii/S1350417724000804?via%3Dihub#ak005 0 d0860fdd-1397-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=201415 Fakulteta za strojništvo Biotehniška fakulteta Medicinska fakulteta Fakulteta za matematiko in fiziko 0 0 0