20.500.12556/RUL-168158 The status of ESCA disease and the disinfection of the scion prior to grafting affect the phenolic composition and phenylpropanoid-related enzymes in the callus of vine hetero-grafts Vegetative propagation of European grapevine (Vitis vinifera L.) requires grafting onto American rootstocks due to susceptibility to phylloxera. However, the grafting yield is compromised by the presence of grapevine trunk diseases (GTDs) such as Esca. This study investigates the phenolic response and enzyme activity in grapevine callus from grafts obtained by scions with different GTD status (healthy, asymptomatic, and symptomatic) treated with different disinfection methods (Beltanol, Beltanol in combination with thermotherapy, Serenade$^®$ ASO, Remedier, BioAction ES, and sodium bicarbonate). Twenty-three phenolic compounds were identified in the graft callus, with flavanols, stilbenes, and condensed tannins predominating. Scion disinfection with BioAction ES led to a significant increase in total phenolic content in the callus, especially in symptomatic scions, for on average 510.3 µg/g fresh weight (FW) higher total phenolic content, compared to grafts where scions were treated with Beltanol. Phenolics such as epicatechin gallate, procyanidin derivatives, and resveratrol hexoside were significantly increased, indicating a strong elicitor effect of BioAction ES. Enzymatic activity analysis showed that the disinfection methods affected the activity of key enzymes involved in the phenylpropanoid metabolic pathway. In particular, BioAction ES significantly increased phenylalanine ammonia lyase (PAL) activity in callus from grafts with healthy scions by 3.4-fold and flavanone 3β-hydroxylase (FHT) activity in callus from grafts with infected scions by 4.9-fold (asymptomatic) and 6.9-fold (symptomatic) compared to callus from grafts with Beltanol-treated scions. The results highlight the potential of environmentally friendly disinfection methods, particularly BioAction ES, in influencing phenolic content and enzymatic activity in graft callus, potentially affecting the success of grapevine grafting. disinfection grapevine trunk diseases grafting phenolic compounds nursery vegetativno razmnoževanje bolezni rastlin vinska trta razkuževanje kap vinske trte cepljenje fenolne spojine true false true Angleški jezik Slovenski jezik Članek v reviji 2025-03-31 17:56:40 2025-03-31 17:56:46 2025-04-01 03:59:51 0000-00-00 00:00:00 2025 0 0 18 str. iss. 4, art. 371 Vol. 11 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.8 2311-7524 10.3390/horticulturae11040371 230862339 horticulturae-11-00371-v2_(1).pdf horticulturae-11-00371-v2_(1).pdf 1 48B40633F609CA3413E8513240F24FAA 456e5ec4496fc72142279adb78fee47a9e3ced09f6917af5ec83f6bb5ee43b13 957d4c6d-0e48-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=201226 https://www.mdpi.com/2311-7524/11/4/371 1 b6d3729e-0e47-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=201225 Biotehniška fakulteta 0 0 0