20.500.12556/RUL-168101 Molecular characterization, virulence, and antimicrobial susceptibility of mycoplasma bovisassociated with chronic mastitis in dairy cows Molekularna karakterizacija, virulenca in protimikrobna občutljivost z mikoplazmo M. bovis povezanega kroničnega mastitisa pri kravah molznicah Mycoplasma bovis (M. bovis) is the most common Mycoplasma species, which has a growing impact on the dairy industry. The purpose of this study was to in-vestigate the molecular characterization, virulence, and antibiotic susceptibility of M. bo-vis isolates from dairy cows with chronic mastitis in Egypt. Eighty-four composite milk samples from mastitic cows were aseptically collected from dairy farms in Egypt. Based on microbiological examination and the polymerase chain reaction (PCR) technique targeting the 16S rRNA, 14 (16.7%) of all milk samples were positive for Mycoplasmaspp. PCR targeting M. bovis-specific gene identified six (7.14%) of the 14 mycoplasma isolates as M. bovis. PCR assays for different virulence genes showed that all M. bovisisolates exhibited the presence of vspA gene, while other virulence genes (uvrC, gap, and p40 pseudogenes) were determined in only two M. bovis isolates (2/6). The 16S rRNA gene phylogenetic analysis demonstrated 100% homology with reference strains of M. bovis isolated from different species and locations. When compared to other iso-lates in the GenBank, the amino acid sequence alignment of our isolate VspA-like pro-tein indicated a distinct mutation. The in vitro antimicrobial susceptibility testing of the six M. bovis isolates in this study to seven antimicrobial drugs revealed that tilmycosin and tylosin had the lowest minimum inhibitory concentrations (MIC) values (≤1 μg/mL), while danofloxacin, streptomycin and florfenicol had the highest MIC values (<4 μg/mL). Nonetheless, this study showed the virulence of M. bovis in dairy cows in Egypt, and macrolides were found to be the most potent compounds in vitro against all tested isolates, but further studies in nationwide surveys are needed. antimicrobial susceptibility chronic mastitis Mycoplasma bovis sequence analysis virulence genes true false true Angleški jezik Slovenski jezik Članek v reviji 2025-03-28 13:43:36 2025-03-28 13:43:40 2025-03-29 04:14:36 0000-00-00 00:00:00 2024 0 0 Str. 271-279 no. 4 Vol. 61 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-01-01 636.2.09:618.19-002:579.887 1580-4003 10.26873/SVR-1882-2024 227464195 Molecular.pdf Molecular.pdf 1 3AC7A7EC20C5DCFC8111C2278B2F243D 484beacddf4991b887ee94f824255b617d1269c4f5a42b01734cec274e02661c 6055af4f-0bd1-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=201117 https://www.slovetres.si/index.php/SVR/article/view/1882/737 1 3e689c73-0bd1-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=201116 Veterinarska fakulteta Slovenian veterinary research 0 0 0