20.500.12556/RUL-168100 PCV2 and PCV3 genotyping in wild boars from Serbia Genotipizacija PCV2 in PCV3 pri divjih prašičih iz Srbije Porcine circoviruses 2 and 3 (PCV2 and PCV3) are known agents of diseases in domestic pigs and wild boars. PCV2 is an economically important pathogen causing porcine circovirus-associated diseases (PCVAD), while the recently discovered PCV3 is associated with similar disorders. Wild boars can serve as a PCV reservoir for domestic pigs, which is a particular risk for pig farms with low biosecurity. Reports of these infections in Serbia are sporadic, and this study was intended as a follow-up to an earlier study. Our aim was to assess the prevalence and genetic characteristics of PCVs circulating in wild boars in a region in north-eastern Serbia with extensive hunting areas. In our study of 103 samples, 17.48% tested positive for PCV2 and 15.53% for PCV3. The low co-infection rates in 2.94% of the PCR-positive samples, suggests these viruses circulate independently. PCV2 prevalence was lower than in our previous study (40.32% out of 124 samples), but the genetic stability of circulating strains was detected with a clear genotype shift towards PCV2d-2. Moreover, this is the first report of PCV3 occurrence in wild boar in Serbia, and the detected strains were grouped into two genotypes: PCV3-1 and PCV3-3c. The PCV3-1 sequences were clustered with German strains, indicating the prevalence of this genotype in Europe. However, no further geographical correlation could be established, as the PCV3-3c representative was separated within the cluster containing Chinese and Indian strains. Furthermore, there was no correlation between PCV positivity and pathological findings in the sampled animals indicating subclinical infection. PCV2 PCV3 genotyping PCR wild boar true false true Slovenski jezik Angleški jezik Članek v reviji 2025-03-28 13:40:10 2025-03-28 13:40:13 2025-03-29 04:14:36 0000-00-00 00:00:00 2024 0 0 Str. 263-269 no. 4 Vol. 61 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-01-01 601.4:575.116.4: 599.731.111 1580-4003 10.26873/SVR-1708-2024 227302403 PCV2_and_PCV3.pdf PCV2_and_PCV3.pdf 1 D3077EBAB7048033AD3E0FA834EF6E53 9c4d380f8e8319858f2da821a65a349a809aa9f98622fc13755feeedd178935d f17a8eec-0bd0-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=201115 https://www.slovetres.si/index.php/SVR/article/view/1708/736 1 c2a6a53f-0bd0-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=201113 Veterinarska fakulteta Slovenian veterinary research 0 0 0