20.500.12556/RUL-168062 Assessing the presence of Stx2e-producing E. coli virotypes in samples of oral fluid of growers and fatteners cross-sectional study Edema disease is a multifactorial infectious disease caused by specific E. coli virotypes possessing fimbriae F18 and toxin Stx2e that cause significant losses in the post-weaning period. The aim of this study was to assess the presence of Stx2e-producing E. coli verotypes in Slovenian commercial pig farms in relation to the biosecurity and technological measures undertaken by the owners. Samples of oral fluid were collected from growers and fatteners at 5–6 weeks, 7–8 weeks, 12 weeks and 14 weeks of age on 37 commercial pig farms, using the Verocheck® diagnostic kit for the real-time PCR detection of Stx2e. The results of RT-PCR and the questionnaire were statistically analyzed. The prevalence of E. coli strains producing Stx2e was 64.9%. Statistically significant association between the prevalence of Stx2e producing E. coli strains and the type of the farm and feed origin was proved. No association was found between prevalence and farm size, presence of quarantine or previous outbreaks of edema disease. None of the studied age groups showed a statistically significant dominance in prevalence compared to other age groups, which contradicts the current theoretical data. Further studies are needed to estimate the proportion of Stx2e produced by the EDEC pathotype compared to other E. coli strains. edema disease oral fluid E. coli prevalence biosecurity true false true Angleški jezik Ni določen Članek v reviji 2025-03-27 11:31:10 2025-03-27 11:31:13 2025-10-24 10:58:49 0000-00-00 00:00:00 2025 0 0 25 str. iss. 3, art. 261 Vol. 14 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579:636.09 2076-0817 10.3390/pathogens14030261 229983491 Cross-Stukelj.pdf Cross-Stukelj.pdf 1 4E99710B77D18914BB64D20FD5834192 0d2b3a9ae9d78a2c3eb60a4b07ed744e02e088d185a8a32bad0d3b7330d0f57a cc666340-0af6-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=201052 https://www.mdpi.com/2076-0817/14/3/261 1 933dbbec-0af5-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=201051 Veterinarska fakulteta Fakulteta za elektrotehniko 0 0 0