20.500.12556/RUL-167951 Konkurenčnost bakterijskih sevov iz rodov Bacteroides in Prevotella v kokulturah pri različnih gojitvenih pogojih Competitiveness of Bacteroides and Prevotella bacterial strains in cocultures under different cultivation conditions V magistrskem delu smo preučevali konkurenčnosti serijskih kokultur več vrst iz rodov Bacteroides in Prevotella pri različnih gojitvenih pogojih. Namen je bil ugotoviti, kateri sevi so bolj konkurenčni pri različnih koncentracijah ksilana in proteinskih hidrolizatov ter ali med izbranimi vrstami obstajajo antagonistične interakcije. Uporabili smo klasične metode striktno anaerobnega dela v kombinaciji z molekularnimi pristopi, kot so sekvenciranje NGS in analiza pridobljenih odčitkov zaporedij gena 16S rRNA. Rezultati so pokazali, da je hitrost rasti posameznih sevov v monokulturah le delno napovedovala prevlado v kokulturah. Poleg tega med izbranimi sevi nismo dokazali antagonizma, zato sklepamo, da je konkurenčnost izbranih sevov odvisna od stopnje učinkovitosti mehanizmov za prevzem in razgradnjo ksilana kot tudi od pretvorbe alternativnih virov ogljika (proteinskih hidrolizatov). V monokulturah so izbrani sevi iz rodu Prevotella, kljub počasni rasti, dosegali višje vrednosti OD600 in tudi prevladali v vseh preizkušenih kokulturah, ne glede na spremembe v količini ksilana, proteinskih hidrolizatov ali vrednosti pH. Na osnovi tega sklepamo, da njihova konkurenčnost temelji tako na učinkovitejših metabolnih mehanizmih kot tudi na boljši prilagodljivosti na spremembe v okolju. The master thesis focuses on the study of the competitiveness of serial co-cultures of several Bacteroides and Prevotella species under different cultivation conditions. The aim of the MSc thesis was to determine which strains are more competitive at different concentrations of xylan and protein hydrolysates and whether antagonistic interactions exist between the selected species. We used classical methods of strictly anaerobic work in combination with molecular approaches such as NGS sequencing and analysis of the obtained 16S rRNA sequence reads. The results showed that the growth rate of individual strains in monocultures only partially predicted their dominance in co-cultures. Moreover, no antagonism was detected between the selected strains, suggesting that the competitiveness of the selected strains depends on the efficiency of the xylan uptake and degradation mechanisms and the conversion of alternative carbon sources (protein hydrolysates). In monocultures, the selected Prevotella strains, despite their slower growth rate, achieved higher OD600 values and dominated in all co-cultures tested, irrespective of changes in xylan and protein hydrolysate concentrations or pH value. Based on these findings, we conclude that their competitiveness is based on more efficient metabolic mechanisms and better adaptability to environmental changes. črevesna mikrobiota Prevotella Bacteroides monokultura kokultura sekvenciranje NGS razgradnja ksilana razgradnja proteinskih hidrolizatov gut microbiota Prevotella Bacteroides monoculture co-culture NGS sequencing xylan degradation degradation of protein hydrolysates true false false [N. Kozoderc] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-03-21 07:15:45 2025-03-21 07:15:46 2025-03-22 04:08:15 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.24/.26:575.112 257175 229840131 46186.pdf 46186.pdf 1 BA11ADAA417EBBDBCDD1D9173C08577B e533d56f64cdd2c45f7c9765e852a6624209d70cc9778b37b171f8f36e22d448 e9db0042-061a-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200887 46187.xlsx 46187.xlsx 1 0067C0870D0FBA5BC9B3BB2466CF2C67 77bc8788cf0be7a6b35b6d510786a8031aad396e1ad95032671bc1704efb79d8 e9db9237-061a-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200888 46188.xlsx 46188.xlsx 1 DC92EE800AD8789225B425B142CD504D 688d12ab6d6cd2aa557a1b2b501df63f963a6db77d5855eb9f60efb983de37d0 e9de471b-061a-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200889 46189.xlsx 46189.xlsx 1 3B1492F87CAD36826CB09E26D20FB8D3 acc8a6b9da54355f690e277e774555c3b34b7dd02a64fb42b2a5b9483bac397a e9de8db7-061a-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200890 46190.xls 46190.xls 1 ECEF265B651D77DD867F8B48CB07B62C 0ab61acc190e55ca4f78511161eaf27e921ee13fac87613a144bd2675f2f0110 e9dec488-061a-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200891 Biotehniška fakulteta 0 0 0