20.500.12556/RUL-167943 Vpliv silicija na rastline, izpostavljene pomanjkanju dostopne vode Influence of silicon on plants exposed to water deficit Namen magistrske naloge je bil raziskati vpliv silicija (Si) na hidroponsko gojen navadni ječmen (Hordeum vulgare L.) v razmerah pomanjkanja vode, simuliranih z dodatkom polietilenglikola (PEG). Rastline so bile izpostavljene štirim različnim tretmajem: Si0 PEG0, Si+ PEG0, Si0 PEG+, Si+ PEG+. Merili smo morfološke (dolžina poganjkov, število listov, sveža in suha masa rastlinskih delov, RWC), fiziološke (fotokemična učinkovitost fotosistema II, prevodnost listnih rež) in biokemijske lastnosti (koncentracija fotosinteznih pigmentov, lipidna peroksidacija, koncentracija elementov Si, P, S, Cl, K in Ca) ječmena. Na podlagi izvedenih meritev in analiz, smo ugotovili, da Si in PEG ter njuna kombinacija, vplivata na določene lastnosti ječmena, izpostavljenega pomanjkanju vode. Dodatek Si v hranilno raztopino (HR) je povečal število zelenih listov in koncentracijo Si v listih ter zmanjšal oksidativno peroksidacijo listov. Sušni stres, simuliran s PEG, je imel večinoma negativen vpliv na izmerjene parametre. Opazili smo, da dodatek PEG v HR ne glede na prisotnost Si, zmanjša število zelenih listov, zmanjša svežo maso listov, stebel in korenin ter suho maso listov rastlin ječmena. Silicij je omilil nekatere negativne učinke sušnega stresa, predvsem na morfološke lastnosti. Za bolj zanesljivo razlago rezultatov, bi bilo potrebno izvesti več meritev posameznih parametrov in preveriti vpliv različnih koncentracij Si in PEG. The purpose of this research was to investigate the effect of silicon (Si) on hydroponically grown barley (Hordeum vulgare L.), under water deficit, simulated using polyethylene glycol (PEG). The plants were grown in four different nutrient solutions: Si0 PEG0, Si+ PEG0, Si0 PEG+ and Si+ PEG+. Morphological traits (shoot length, number of leaves, fresh and dry biomass, RWC), physiological traits (photochemical efficiency of photosystem II, stomatal conductance), and biochemical traits (concentration of photosynthetic pigments, lipid peroxidation, and elemental concentrations of Si, P, S, Cl, K, and Ca) were measured. Silicon, PEG, and their combination influenced certain traits of barley exposed to water deficit. Addition of Si to the nutrient solution increased the number of green leaves and the concentration of Si in leaves, and reduced oxidative lipid peroxidation in leaves. Water stress, simulated with PEG, predominantly had a negative impact on the measured parameters. It was observed that the addition of PEG to the nutrient solution, regardless of the presence of Si, resulted in a reduced number of green leaves and decreased fresh biomass of leaves, stems, and roots, as well as reduced dry biomass of barley leaves. Silicon mitigated some of the adverse effects of water stress, particularly in morphological traits. Further analyses would be required for a more reliable interpretation of the results. It would be necessary to perform more measurements of individual parameters and to check the effect of different concentrations of Si and PEG on barley plants. Ječmen (Hordeum vulgare L.) silicij polietilenglikol hidroponika pomanjkanje dostopne vode Barley (Hordeum vulgare L.) silicon polyethyleneglycol hydroponics water deficit true false false [D. Šarman] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-03-21 07:15:06 2025-03-21 07:15:18 2025-03-22 04:08:13 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 581.5:633.16:546.28(043.2) 257170 229783299 46115.pdf 46115.pdf 1 7717040A26C329FF660E8517B6D83CDC b59e6bedb401a78a9086c479498799f9362c91a96680800658e6ed89d47f06e1 db891024-061a-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200876 Biotehniška fakulteta 0 0 0