20.500.12556/RUL-167802 Nisin-loaded gelatin microparticles for the enhanced bioactivity of bacterial nanocellulose Bacterial nanocellulose (BnC) is of immense importance in medicine, although its lack of bioactivity present intriguing issue. We propose a method to modify BnC with gelatin and nisin biomolecules, and explore their synergistic effect on the antimicrobial activity. Gelatin microparticles (without/with nisin loading) with a size ~0.5 μm and ~1.3 μm were prepared by spray drying and stabilised by dehydrothermal treatment. Modified BnC-based membranes supported the formation of biologically relevant minerals and were non-cytotoxic to human gingival fibroblast cells (HGF). The presence of gelatin microparticles improved the viability of HGF by approximately 20 %, due to the effect of gelatin alone, independent of the addition of nisin. BnC coated with a nisin/gelatin solution reduces the viability of HGF by about 20 %, but this negative effect is not observed by nisin coated gelatin microparticles. The cell viability of BnC membranes was above 90 % in both porcine and human urothelial cells. The antimicrobial activity study confirmed an inhibitory effect of membranes modified with nisin-coated microparticles or a gelatin/nisin solution against Staphylococcus aureus at a non-cytotoxic nisin dose (150 μg/mL). The study demonstrates the structural effects of gelatin and gelatin/nisin mixtures on the bioactivity of BnC and provides a rationale for the modification procedure. bacterial nanocellulose gelatin nisin spray-drying antimicrobial function bioactivity bakterijska nanoceluloza želatina nizin sušenje z razprševanjem protimikrobno delovanje bioaktivnost true false true Angleški jezik Slovenski jezik Članek v reviji 2025-03-12 15:23:48 2025-03-12 15:23:52 2025-03-13 04:15:53 0000-00-00 00:00:00 2025 0 0 15 str. pt. 1, art. 141203 Vol. 305 May 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577:543.2/.9 1879-0003 10.1016/j.ijbiomac.2025.141203 228411907 1-s2.0-S0141813025017520-main.pdf 1-s2.0-S0141813025017520-main.pdf 1 EBDEC97814D0DC00534BE326ABAD53AB 9f8676dc51034210bff902425e8e3ef482bb43c336d9cfbed77fa07001e2459c fab5f123-ff4c-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200587 https://www.sciencedirect.com/science/article/pii/S0141813025017520 0 d868569b-ff4c-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200586 Medicinska fakulteta 0 0 0