20.500.12556/RUL-167765 Clostridioides difficile in raw mechanically separated poultry meat and pasteurized product made from contaminated meat Objectives: Clostridioides difficile (C. difficile) is an important foodborne pathogen found in a wide range of products. This study investigated the occurrence of C. difficile in mechanically separated chicken and turkey meat (MSM) and in pasteurized products made from contaminated MSM. Methods: The presence of C. difficile was analysed in 56 MSM samples (32 from turkey and 24 from chicken) and in six pasteurized meat products made from raw meats previously identified as C. difficile-positive. After enrichment, detection was performed by real-time PCR, and culture on selective media for C. difficile. The isolated strains were then characterized by PCR-ribotyping, toxinotyping, and whole-genome sequencing (WGS). Results: C. difficile was detected in 16.1 % of MSM samples via real-time PCR, with a 10.7 % isolation rate. One pasteurized product also tested positive. The six isolates obtained displayed diverse PCR-ribotypes, five of which were toxigenic. Notably, the PCR-ribotypes and sequence types in the pasteurized product differed from those identified in the raw meat used for its production. Conclusions: The presence of C. difficile in raw and subsequently pasteurized meat product indicates that the pathogen can survive the pasteurization process and may be present in such products. Clostridioides difficile mechanically separated poultry meat pasteurized products food whole-genome sequencing true false true Angleški jezik Ni določen Članek v reviji 2025-03-11 10:16:48 2025-03-11 10:16:54 2025-03-13 15:35:16 0000-00-00 00:00:00 2025 0 0 5 str. Vol. 92, art. 102946 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:577 1095-8274 10.1016/j.anaerobe.2025.102946 228527619 Clostridioides-Henigman.pdf Clostridioides-Henigman.pdf 1 71DB2080BA622A279E5E758A2CC15867 14e81e3967c662d05e16c7f9b127db38951d6c021f4105dc2d832d72a93efdd6 6780a114-fe5a-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200518 https://www.sciencedirect.com/science/article/pii/S1075996425000095 0 9a8db172-fe58-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200515 Veterinarska fakulteta 0 0 0