20.500.12556/RUL-167756 High-field diffusion tensor imaging of median, tibial, and sural nerves in type 2 diabetes with morphometric analysis Background and Purpose: The primary objective was to compare diffusion tensor imaging (DTI) scalar parameters of peripheral nerves between subjects with type 2 diabetes mellitus (T2DM) and those without diabetes. Secondarily, we aimed to correlate DTI scalar parameters with nerve morphometric properties. Methods: Median, tibial, and sural nerves were harvested from 34 male cadavers (17 T2DM, 17 nondiabetic). Each nerve was divided into three segments. The initial segment was scanned using 9.4 Tesla MRI system (three-dimensional pulsed-gradient spin-echo sequence). DTI scalars were calculated from region-average diffusion-weighted signals. Second segment was optically cleared, acquired with optical projection tomography (OPT), and analyzed for morphometrical properties. Toluidine-stained sections were prepared from last segment, and axon- and myelin-related properties were evaluated. Results: DTI scalar parameters of median and tibial nerves were comparable between the groups, while sural nerves of T2DM exhibited on average 41% higher mean diffusivity (MD) (p = 0.03), 38% higher radial diffusivity (RD) (p = 0.03), and 27% lower fractional anisotropy (FA) (p = 0.005). Significant differences in toluidine-evaluated parameters of sural nerves were observed between the groups, with a positive correlation between FA with fiber density (p = 0.0001) and with myelin proportion (p < 0.0001) and an inverse correlation between RD and myelin proportion (p = 0.003). OPT-measured morphometric properties did not correlate with DTI scalar parameters. Conclusions: High-field DTI shows promise as an imaging technique for detecting axonal and myelin-related changes in small sural nerves ex vivo. The reduced fiber density and decreased myelin content, which can be observed in T2DM, likely contribute to observed FA reduction and increased MD/RD. diffussion tensor fractional anisotropy histology magnetic resonance imaging microscopy optical projection tomography difuzijski tenzor frakcijska anizotropija histologija slikanje z magnetno resonanco mikroskopija optična projekcijska tomografija true false true Angleški jezik Slovenski jezik Članek v reviji 2025-03-10 15:04:53 2025-03-10 15:05:00 2025-03-11 04:03:06 0000-00-00 00:00:00 2025 0 0 11 str. iss. 1, art. e70025 Vol. 35 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-07 1552-6569 10.1111/jon.70025 228292611 Journal_of_Neuroimaging_-_2025_-_Pusnik_-_High‐Field_Diffusion_Tensor_Imaging_of_Median__Tibial__and_Sural_Nerves_in_Type_2.pdf Journal_of_Neuroimaging_-_2025_-_Pusnik_-_High‐Field_Diffusion_Tensor_Imaging_of_Median__Tibial__and_Sural_Nerves_in_Type_2.pdf 1 4107F848464A08DB22932B3912B1ED14 7b8bbdfc2b41ed6723bf2dd14cbdd1f72d1a27ada6f3b241741e6015b19998d1 dd25ae15-fdb8-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200506 https://onlinelibrary.wiley.com/doi/10.1111/jon.70025 1 b09698e3-fdb7-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200505 Medicinska fakulteta 0 0 0