20.500.12556/RUL-167736 Responses of parasitic nematodes to volatile organic compounds emitted by Brassica nigra roots Parasitic nematodes, particularly those in the Rhabditidae family, are vital components of belowground ecosystems, contributing to pest regulation and sustainable agriculture. This study investigated the chemotactic responses of three nematode species—Phasmarhabditis papillosa, Oscheius myriophilus, and O. onirici—to volatile organic compounds (VOCs) emitted by Brassica nigra roots under herbivory by Delia radicum larvae. Using a chemotaxis assay, the effects of five VOCs (dimethyl sulfide, dimethyl disulfide, allyl isothiocyanate, phenylethyl isothiocyanate, and benzonitrile) were tested at two concentrations (pure and 0.03 ppm) and two temperatures (18 ◦C and 22 ◦C). The results revealed that VOCs and temperature significantly influenced nematode responses, while nematode species and VOC concentration showed limited effects. Benzonitrile consistently demonstrated strong chemoattractant properties, particularly for O. myriophilus and O. onirici. Conversely, allyl isothiocyanate exhibited potent nematicidal effects, inhibiting motility and causing mortality. Dimethyl disulfide and dimethyl sulfide elicited moderate to strong attractant responses, with species- and temperature-dependent variations. Significant interactions between VOCs, temperature, and nematode species highlighted the complexity of these ecological interactions. These findings emphasize the ecological roles of VOCs in mediating nematode behavior and their potential applications in sustainable pest management. Benzonitrile emerged as a promising candidate for nematode-based biocontrol strategies, while allyl isothiocyanate showed potential as a direct nematicidal agent. The study underscores the importance of integrating chemical cues into pest management systems to enhance agricultural sustainability and reduce reliance on chemical pesticides. parasitic nematodes Phasmarhabditis papillosa Oscheius myriophilus Oscheius onirici chemotaxis volatile organic compounds benzonitrile phenylethyl isothiocyanate belowground interactions parazitske ogorčice polži kemotaksija hlapne organske spojine hlapni izločki obramba rastline biotično varstvo rastlin benzonitril feniletil izotiocianat true false true Angleški jezik Slovenski jezik Članek v reviji 2025-03-10 11:41:30 2025-03-10 11:41:36 2025-03-11 04:03:04 0000-00-00 00:00:00 2025 0 0 14 str. iss. 3, art. 664 Vol. 15 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 632 2073-4395 10.3390/agronomy15030664 228412163 Responses_of_Parasitic_Nematodes_to_Volatile_Organic_Compounds_Emitted_by_Brassica_nigra_Roots.pdf Responses_of_Parasitic_Nematodes_to_Volatile_Organic_Compounds_Emitted_by_Brassica_nigra_Roots.pdf 1 1B521ADE3D86382FD8BB5722E0A17B8F b3af159d20c17799b4d2b07e4765091d2f472ce345b7e17fd3aaa33cf801996f ac565ef4-fd9b-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200470 https://www.mdpi.com/2073-4395/15/3/664 1 466b544f-fd9b-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200468 Biotehniška fakulteta 0 0 0