20.500.12556/RUL-167607 Urinary metabolomic profile in children with autism spectrum disorder Autism spectrum disorder (ASD) has been associated with disruptions in tryptophan (TRP) metabolism, affecting the production of key neuroactive metabolites. Investigating these metabolic pathways could yield valuable biomarkers for ASD severity and progression. We included 44 children with ASD and 44 healthy children, members of the same family. The average age in the ASD group was 10.7 years, while the average age in the control group was 9.4 years. Urinary tryptophan metabolites were quantified via liquid chromatography—mass spectrometry operating multiple reaction monitoring (MRM). Urinary creatinine was analyzed on an Advia 2400 analyzer using the Jaffe reaction. Statistical comparisons were made between ASD subgroups based on CARS scores. Our findings indicate that children with ASD have higher TRP concentrations (19.94 vs. 16.91; p = 0.04) than their siblings. Kynurenine (KYN) was found at higher levels in children with ASD compared to children in the control group (82.34 vs. 71.20; p = 0.86), although this difference was not statistically significant. The ASD group showed trends of higher KYN/TRP ratios and altered TRP/ indole-3-acetic acid (IAA) and TRP/5-hydroxyindoleacetic acid (5-HIAA) ratios, correlating with symptom severity. Although the numbers of the two groups were different, our findings suggest that mild and severe illnesses involve separate mechanisms. However, further comprehensive studies are needed to validate these ratios as diagnostic tools for ASD. autism spectrum disorder tryptophan kynurenine CARS triptofan kinurenin CARS nevroaktivni metaboliti motnje avtističnega spektra true false true Angleški jezik Slovenski jezik Članek v reviji 2025-03-04 09:25:09 2025-03-04 09:25:16 2025-03-05 04:03:06 0000-00-00 00:00:00 2025 0 0 18 str. iss. 5, [article no.] 2254 Vol. 26 Mar. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.896 1422-0067 10.3390/ijms26052254 227890947 ijms-26-02254-v2.pdf ijms-26-02254-v2.pdf 1 09F15BFDD1F338920F88E766CD40485F 3a49ce2432f4d82152d6186ca93b6c5bb7285518b8f06a8113d810fddc07b8f1 994ca9be-f8d1-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200275 https://www.mdpi.com/1422-0067/26/5/2254 1 465ffe00-f8d1-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200274 Fakulteta za farmacijo Fakulteta za matematiko in fiziko Biotehniška fakulteta 0 0 0