20.500.12556/RUL-167301 Vpliv profaga na larvicidnost bakterije Bacillus thuringiensis T0131 za vrsti Drosophila suzukii in Galleria mellonella magistrsko delo The effect of prophage on Bacillus thuringiensis T0131 larvicidal activity against Drosophila suzukii and Galleria mellonella M. Sc. Thesis Bakteriofag (fag) GIL01 okužuje bakterijo Bacillus thuringiensis serovar israelensis. Za izolate te bakterije je značilno, da tekom sporulacije lahko sintetizirajo kristalne toksine, ki so larvicidni. Pripravki izolatov te bakterije so najpogosteje uporabljeni biopesticidi na planetu. V večini larvicidnih izolatov te bakterije se nahaja genom faga (profag) GIL01. Fag GIL01 je temperatni fag, ki v primeru poškodb bakterijske DNA preide v litični cikel. V študiji smo uporabili izolat B. thuringiensis serovar israelensis T0131, ki je bil izoliran iz zemlje v Braziliji. V nasprotju z drugimi naravnimi izolati B. thuringiensis serovar israelensis, izolat T0131 nima profaga GIL01. V nalogi smo uspeli pridobiti lizogeni sev T0131-GIL01. To nam je omogočalo analizo ali prisotnost profaga GIL01 v izolatu T0131 vpliva na preživetje ličink voščene vešče (Galleria mellonella) ali ličink plodove vinske mušice (Drosophila suzukii). Z metodami prisilnega hranjenja in subkutanega injiciranja bakterijskih spor nismo uspeli nedvoumno ugotoviti, ali profag GIL01 v izolatu T0131 vpliva na preživetje ličink voščene vešče. Z metodo prisilnega hranjenja pa smo ugotovili, da izolirani fag GIL01 lahko izboljša preživetje voščene vešče. Z dodajanjem spor v gojišče smo ugotovili, da spore sevov T0131, in T0131 z GIL01 v lizogenem ciklu ne vplivajo na stopnjo smrtnosti ali hitrosti razvoja ličink plodove vinske mušice od jajčeca do odrasle muhe. The bacteriophage (phage) GIL01 infects the bacteria Bacillus thuringiensis serovar israelensis. A distinct characteristic of isolates of this bacterium is the fact that they produce larvicidal crystal toxins during sporulation. Commercial products based on different B. thuringiensis isolates are the most used biopesticides in the world. Most isolates of B. thuringiensis serovar israelensis already possess the genome of the phage (prophage) GIL01. Phage GIL01 is a temperate phage, that switches to a lytic cycle, when bacterial DNA damage occurs. In our studies we used the isolate B. thuringiensis serovar israelensis T0131, which was isolated from soil in Brazil. In contrast to most isolates of B. thuringiensis serovar israelensis, T0131 does not include the prophage GIL01. During our study we were able to produce a lysogenic strain T0131-GIL01. This enabled us to investigate whether the presence of GIL01 in T0131 effects the survivability of Galleria mellonella larvae, or the larvae of Drosophila suzukii. Using the methods of forced feeding and subcutaneous injection of bacterial spores, we were not able to determine whether the presence of GIL01 in T0131 effects the survivability of G. mellonella larvae. Forced feeding with pure GIL01 however, significantly increased the survival rate of G. mellonella. By adding spores of T0131 and T0131 with GIL01 in a lysogenic cycle to the growth medium, we determined that none of the tested strains effects the survivability or development time of D. suzukii from egg to adult fly. bakteriofag GIL01 Bacillus thuringiensis israelensis T0131 Galleria mellonella Drosophila suzukii larvicidnost spore. bacteriophage GIL01 Bacillus thuringiensis israelensis T0131 Galleria mellonella Drosophila suzukii larvicide spores. true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-02-14 07:15:55 2025-02-14 07:16:00 2025-02-15 04:13:18 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 256054 226136835 45663.pdf 45663.pdf 1 A4C601AA9AEBB4AA5D7B0EE3319B4E0C 51900d418de53d084649a5e4c613ee7a76359cd789a71e1ebb1e981d80f73e27 4679edd6-ea9a-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=199640 Biotehniška fakulteta 0 0 0