20.500.12556/RUL-167019 Advancing microplastic detection in zebrafish with micro computed tomography a novel approach to revealing microplastic distribution in organisms The analysis of microplastics with current spectroscopic and pyrolytic methods is reaching its limits, especially with regard to detailed spatial distribution in biological tissues. This limitation hampers a comprehensive understanding of the effects of microplastics on organisms. Therefore, there is a pressing need to expand the analytical approaches to study microplastics in biota. In this context, the aim of this study was to test the applicability of non-destructive 3D imaging using X-ray micro-computed tomography (microCT) for the detection of microplastics in fish. Zebrafish (Danio rerio) were gavaged with polyethylene spherical microplastics (30–110 μm) and the distribution of microplastics in the gut was investigated using microCT. The results showed that the particle size distribution determined by microCT closely matched the data from conventional laser diffraction analysis. In addition, microCT was able to detect microplastics in spiked fish tissue and provide precise localization data by tracing particles of known type and shape. MicroCT offers a novel approach for tracking microplastics in organisms and enables accurate sizing without compromising the integrity of the tissue under investigation. It therefore represents a valuable addition to spectroscopic methods, which are widely used for the detection of microplastics based on their chemical composition but do not provide data on their spatial distribution. microplastics X-ray computed tomography environment plastic pollution imaging mikroplastika rentgenska računalniška tomografija okolje onesnaženje plastiko slikanje true false true Angleški jezik Slovenski jezik Članek v reviji 2025-02-04 10:25:00 2025-02-04 10:25:01 2025-02-05 04:01:46 0000-00-00 00:00:00 2025 0 0 12 str. Vol. 488, art. 137442 5 May 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 504.5:678 0304-3894 10.1016/j.jhazmat.2025.137442 224993539 1-s2.0-S0304389425003541-main.pdf 1-s2.0-S0304389425003541-main.pdf 1 5EE1E769B92A473E4FF05C16441712D7 ff346f0e3a43f25e59facb9e42e8a4a3b5de9d775fd072f83d1302ca804399a4 0b4f9fc7-e2da-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=199120 https://www.sciencedirect.com/science/article/pii/S0304389425003541 1 12957bc5-e2d9-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=199119 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0