20.500.12556/RUL-166917 TLR10 overexpression modulates immune response in A549 lung epithelial cells challenged with SARS-CoV-2 S and N proteins Toll-like receptors (TLRs) play an important role in the recognition of viral particles and activation of the innate immune system, but their role in SARS-CoV-2 infection is still poorly characterized. In the present study, we investigated the role of Toll-like receptor 10 (TLR10) in modulating the immune response during SARS-CoV-2 infection. The results showed that overexpression of TLR10 in A549 lung epithelial cells, immunostimulated with SARS-CoV-2 proteins S and N mainly downregulated proinflammatory cytokines and interferons and affected gene expression in the cocultured THP-1 monocytes. Our results suggest that TLR10 could mediate the extent of SARS-CoV-2 infection by downregulating the release of inflammatory cytokines and chemokines such as CXCL10, IL6, IL8, and IFNβ. Modulation of TLR10 expression could have implications for the treatment of patients with severe COVID-19, in whom excessive inflammation leading to the development of acute respiratory distress syndrome (ARDS) is a key feature. However, further research is needed to fully understand the impact of modulating TLR10 expression on the antiviral response and the overall balance of the immune response during SARS-CoV-2 infection. SARS-CoV-2 COVID-19 Toll-like receptor 10 interferons proinflammatory cytokines innate immunity imunologija pljuča epitelne celice vnetje TLR10 COVID-19 SARS-CoV-2 true false true Angleški jezik Slovenski jezik Članek v reviji 2025-01-31 04:01:29 2025-01-31 14:23:11 2025-02-01 04:06:23 0000-00-00 00:00:00 2025 0 0 11 str. art. 1490478 Vol. 15 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.27 1664-3224 10.3389/fimmu.2024.1490478 223003139 fimmu-1-1490478.pdf fimmu-1-1490478.pdf 1 0E7812F5D0EF471A2CDE3BB637A77A23 0d8ea4b4295b62f6398ae8429ee284086bbc257b8723e9703a24e3f9b0c44798 6d25fa58-dfd5-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=199007 https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1490478/full 1 d9139b74-df7e-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198978 Biotehniška fakulteta 0 0 0