20.500.12556/RUL-166863 Biophysics and electrophysiology of pulsed field ablation in normal and infarcted porcine cardiac ventricular tissue Pulsed Field Ablation (PFA) is a new ablation method being rapidly adopted for treatment of atrial fibrillation, which shows advantages in safety and efficiency over radiofrequency and cryo-ablation. In this study, we used an in vivo swine model (10 healthy and 5 with chronic myocardial infarct) for ventricular PFA, collecting intracardiac electrograms, electro-anatomical maps, native T1-weighted and late gadolinium enhancement MRI, gross pathology, and histology. We used 1000–1500V pulses, with 1–16 pulse trains to vary PFA dose. Lesions were assessed at 24 h, 7 days, and 6 weeks in healthy and at 48 h in infarcted ventricles. Comparisons of lesion sizes using a numerical model enabled us to determine lethal electric field thresholds for cardiac tissue and its dependence on the number of pulse trains. Similar thresholds were found in normal and infarcted hearts. Numerical modeling and temperature-sensitive MRI confirmed the nonthermal nature of PFA, with less than 2% of a lesion’s volume at the highest dose used being attributed to thermal damage. Longitudinal cardiac MRI and histology provide a comprehensive description of lesion maturation. Lesions shrink between 24 h and 7 days post-ablation and then remain stable out to 6 weeks post-ablation. Periprocedural electrograms analysis yields good correlation with lesion durability and size ventricular arrhythmias electroporation pulsed filed ablation srčne aritmije elektroporacija katetrska ablacija true false true Angleški jezik Slovenski jezik Članek v reviji 2025-01-28 10:31:43 2025-01-28 10:31:48 2025-06-30 13:21:58 0000-00-00 00:00:00 2024 0 0 22 str. 14, art. 32063 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 004.93:616.13-007.64:537.635 2045-2322 10.1038/s41598-024-83683-y 221586947 s41598-024-83683-y.pdf s41598-024-83683-y.pdf 1 7704E45DDE9FED39A231026A0903D187 a76eb112123b65e5d68f633cba3608e9e1da73295e0407f41e699a8ee65ea21f 46c3597e-dd5a-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198823 https://www.nature.com/articles/s41598-024-83683-y 1 e3ca0ad9-dd59-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198822 Fakulteta za elektrotehniko 0 0 0