20.500.12556/RUL-166741 Pharmacokinetics of single dose levobupivacaine after peri-incisional subcutaneous infiltration in anaesthetized domestic pigs Increasing use of pigs as models in translational research, and growing focus on animal welfare are leading to better use of effective analgesics and anaesthetics when painful procedures are performed. However, there is a gap in basic knowledge such as pharmacokinetics of different anaesthetics in these species. The main objective of our study was to determine the pharmacokinetics of levobupivacaine in domestic pigs. Twelve female grower pigs weighing 31.17 ± 4.6 kg were subjected to general anaesthesia and experimental surgery, at the end of which they received 1 mg/kg levobupivacaine via peri-incisional subcutaneous infiltration. Plasma samples were collected before administration of levobupivacaine and at 0.5, 1, 2, 4, 8, 12, 24 and 48 h thereafter. Concentrations of levobupivacaine were determined by liquid chromatography coupled with tandem mass spectrometry. Following single dose of levobupivacaine, all animals had measurable plasma concentrations 0.5 h after drug administration, with most peak concentrations observed at the 1-h time point. In all 12 animals, levobupivacaine was below the limit of quantification 48 h after drug administration. The mean maximum plasma concentration, area under the curve and half-life were determined to be 809.98 μg/l, 6552.46 μg/l h and 6.25 h, respectively. Plasma clearance, volume of distribution and weight-normalized volume of distribution were 4.41 l/h, 35.57 l and 1.23 l/kg, respectively. Peak plasma concentrations in our study were well below concentrations that were found to produce toxicity in pigs. bupivacaine levobupivacaine anaesthetic local anaesthesia analgesia pharmacokinetics pigs porcine true false true Angleški jezik Ni določen Članek v reviji 2025-01-23 14:02:50 2025-01-23 14:02:54 2025-01-24 04:05:30 0000-00-00 00:00:00 2024 0 0 Str. 602-611 no. 6 Vol. 58 2024 0000-00-00 PostprintKoncna NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.4.09:616:615 1758-1117 10.1177/00236772241259618 204676867 Pharmacokinetics_proof.pdf Pharmacokinetics_proof.pdf 1 4FDFD987C06D4F9AD9CABFB73E09741C b70d1a7fdddda3c1846fec818c833e5dd5b74a5b6727700a9307b6123606bf4b d6aa23e0-d98a-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198581 https://journals.sagepub.com/doi/10.1177/00236772241259618 1 91396def-d989-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198580 Veterinarska fakulteta Medicinska fakulteta 0 0 0