20.500.12556/RUL-166683 Effect of antioxidant-rich kindergarten meals on oxidative stress biomarkers in healthy 5–6-year-old children a randomized controlled trial As children spend up to 9 h a day in kindergarten, the main purpose of our study was to evaluate the effect of antioxidant-rich kindergarten meals on oxidative stress biomarkers (OSBs) in healthy children. In the randomized control trial with a follow-up, healthy 5–6-year-old children from six kindergartens were randomly divided into a prototype group (PG, n = 40) and a control group (CG, n = 17). PG followed a 2-week antioxidant-rich kindergarten meal plan (breakfast, lunch, and two snacks), and CG followed their standard kindergarten meal plans. Outside the kindergartens, participants ate as usual. We used a consecutive 7-day dietary record inside and outside the kindergarten and the national dietary assessment tool OPEN to assess the total dietary antioxidant capacity (dTAC) of the consumed foods. Malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), and four F2-isoprostane were measured in fasting urine on days 1 and 15. We also measured total antioxidant power (PAT) and hydroperoxides (d-ROMs) in fasting serum on day 15 and obtained the value of the oxidative stress index (OSI). We used a Welch two-sample t-test and multiple regression analysis to compare the prototype and control groups and a nonparametric Wilcoxon signed rank exact test to compare pre- and post-intervention results in urine. Antioxidant-rich kindergarten meals contributed to a significantly (p < 0.05) higher intake of dTAC in PG participants compared to standard meals in CG participants (8.6 vs. 2.8 mmol/day). We detected a negative correlation between dTAC intake and d-ROMs and between dTAC intake and OSI (r =  − 0.29, p = 0.043 and r =  − 0.31, p = 0.032, respectively). A significant decrease in urinary 8-iso-15-prostaglandin-F-2 alpha was detected in PG participants between days 1 and 15; however, no other intra-individual significant differences in urinary OSBs were found. antioxidant-rich diet oxidative stress biomarkers dietary antioxidant capacity reactive oxygen metabolites F2-isoprostanes kindergarten diet prehrana otrok predšolski otroci vrtec dnevni obroki antioksidanti true false true Angleški jezik Slovenski jezik Članek v reviji 2025-01-21 13:06:48 2025-01-21 13:06:56 2025-01-22 03:54:03 0000-00-00 00:00:00 2024 0 0 Str. 3085-3094 iss. 7 Vol. 183 Jul. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 613.2-053.2:641.1 0340-6199 10.1007/s00431-024-05576-6 197529347 s00431-024-05576-6_(1).pdf s00431-024-05576-6_(1).pdf 1 F3489832DF3F35FEF428C845F4D3660A 7f5cc3107b7eb4d5e49572119a10ac1ae7b37347c7f1e93003e554a97a7b6121 a2485c9b-d7ef-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198472 https://link.springer.com/article/10.1007/s00431-024-05576-6 1 6f1716cd-d7ef-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198471 Biotehniška fakulteta Medicinska fakulteta 0 0 0