20.500.12556/RUL-166648 Preparation of dried nanoemulsion formulation by electrospinning Dry eye disease is a multifactorial condition characterized by a loss of homeostasis of the tear film. Among the various treatment approaches, the application of ophthalmic oil-in-water nanoemulsions with incorporated anti-inflammatory drugs represents one of the most advanced approaches. However, the liquid nature of nanoemulsions limits their retention time at the ocular surface. Transforming the nanoemulsions into a dry form that would disperse rapidly in the tear fluid would improve the retention of the drug at the ocular surface. The aim of this study was to investigate electrospinning as a method for the preparation of a solid eye preparation based on nanoemulsion loaded with the anti-inflammatory drug loteprednol etabonate. Four nanoemulsions differing in oil-to-surfactant ratios were incorporated in hydrophilic nanofibers based on polyethylene oxide, poloxamer 188, and Soluplus®. The dried nanoemulsions in the form of nanofibers dispersed readily on contact with aqueous medium, resulting in a dispersion of nanometre-sized droplets with average size comparable to the average droplet size of the initial nanoemulsions. A rheological study revealed the predominant elastic behavior of the dispersed nanofibers, which indicates the formation of a weak gel after the dispersion of the dried nanoemulsion in tear fluid at the ocular surface. The biocompatibility of the dried nanoemulsions in the form of nanofibers after a single and multiple-dose application was confirmed using the 3D HCE-T model of the stratified epithelium of the human cornea, suggesting that this innovative solid eye preparation could represent a new approach to the treatment of dry eye disease. dry eye disease drying electrospinning eye preparations nanoemulsions nanofibers bolezen suhega očesa sušenje elektropredenje pripravki za oči nanoemulzije nanovlakna nanomateriali true false true Angleški jezik Slovenski jezik Članek v reviji 2025-01-20 11:46:14 2025-01-20 11:46:23 2025-01-21 03:59:14 0000-00-00 00:00:00 2025 0 0 14 str. Vol. 206, art. 107015 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.017-022.513.2 0928-0987 10.1016/j.ejps.2025.107015 222894595 1-s2.0-S0928098725000144-main.pdf 1-s2.0-S0928098725000144-main.pdf 1 AF36F82CFB4F5EA9DC9E80D158D6A1F2 924556583bc33c15e568c93c71cea4db939b77a2c2af14aba99c0c7ba3a523d8 86cbe9bf-d71b-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198399 https://www.sciencedirect.com/science/article/pii/S0928098725000144 1 05f21cb5-d71b-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198398 Fakulteta za farmacijo 0 0 0