20.500.12556/RUL-166601 Transcriptomic analysis of polysaccharide utilization loci reveals substrate preferences in ruminal generalists Segatella bryantii TF1-3 and Xylanibacter ruminicola KHP1 Bacteria of the genera Xylanibacter and Segatella are among the most dominant groups in the rumen microbiota. They are characterized by the ability to utilize different hemicelluloses and pectin of plant cell-wall as well as plant energy storage polysaccharides. The degradation is possible with the use of cell envelope bound multiprotein apparatuses coded in polysaccharide utilization loci (PULs), which have been shown to be substrate specific. The knowledge of PUL presence in rumen Xylanibacter and Segatella based on bioinformatic analyses is already established and transcriptomic and genetic approaches confirmed predicted PULs for a limited number of substrates. In this study, we transcriptomically identified additional different PULs in Xylanibacter ruminicola KHP1 and Segatella bryantii TF1-3. We also identified substrate preferences and found that specific growth rate and extent of growth impacted the choice of substrates preferentially used for degradation. These preferred substrates were used by both strains simultaneously as judged by their PUL upregulation. Lastly, β-glucan and xyloglucan were used by these strains in the absence of bioinformatically and transcriptomically identifiable PUL systems. Segatella Xylanibacter Prevotella transcriptomics polysaccharide utilization locus rumen mikrobiologija vamp mikrobiota Segatella Xylanibacter Prevotella genetika transkriptomika polisaharidi true false true Angleški jezik Slovenski jezik Članek v reviji 2025-01-17 14:43:59 2025-01-17 14:44:01 2025-01-19 03:25:30 0000-00-00 00:00:00 2024 0 0 15 str. art. 495 Vol. 25 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579:575 1471-2164 10.1186/s12864-024-10421-z 196467203 s12864-024-10421-z.pdf s12864-024-10421-z.pdf 1 53666B4751A6E97D699ADEFD06328B77 a38b849fca5c2eddce47dfab3ca7caa792c4a4f15b0e8943c0772345e1a7b919 ab67500d-d4d8-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198336 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10421-z 1 5bb3b3f8-d4d8-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198335 Biotehniška fakulteta 0 0 0