20.500.12556/RUL-166598 Dielectric barrier discharge air plasma as a comprehensive disinfection approach to E. coli and S. aureus biofilm management This study aimed to assess the potential of Dielectric Barrier Discharge (DBD) cold atmospheric air plasma for E. coli and S. aureus biofilm management. The bacterial biofilms were grown on stainless-steel coupons and treated with plasma for 1 and 5 min. The results indicate the reduction of E. coli and S. aureus up to 4.3 log CFU ml$^{−1}$ and 3.3 log CFU ml$^{−1}$ , respectively. The optical emission spectroscopy (OES) shows a possible presence of OH and O species as well as the abundance of N$_2$ and N$_2^+$ features in the plasma. Furthermore, Fourier Transform Infrared Spectroscopy (FTIR) revealed changes in wavelengths that are specific for carbohydrates, lipids and proteins when biofilms are treated with plasma for 5 min. This effect was also confirmed by x-ray photoelectron spectroscopy (XPS) as carbon species were decreased after plasma treatment whereas phosphorus and potassium were increased due to release from destructed cell wall. Furthermore, confocal laser scanning microscopy (CLSM) shows the effect of removing biofilm from the surface, where this effect was the most predominant for 5 min plasma treatment. All in all, our study demonstrates that DBD atmospheric air plasma can effectively reduce biofilm viability due to the presence of reactive nitrogen and possible oxygen species within a phase. Moreover, the applied treatment resulted in the successful eradication of biofilms on the surface of stainless-steel coupons. bacterial biofims E. coli S. aureus spectroscopy atmospheric air plasma hygiene dielectric barrier discharge cold atmospheric plasma biofilms reactive species bacteria true false true Angleški jezik Ni določen Članek v reviji 2025-01-17 13:26:21 2025-01-17 13:26:22 2026-01-27 04:23:10 0000-00-00 00:00:00 2025 0 0 10 str. no. 2, art. 025015 Vol. 100 Feb. 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 543.428.3:628.353.153 1402-4896 10.1088/1402-4896/ada4e7 222879491 Udachin_2025_Phys._Scr._100_025015.pdf Udachin_2025_Phys._Scr._100_025015.pdf 1 C8A0FD663F49B0C886B62AE67161BC0D 22a242ef273814281e3767ab2adef10b078f027df408a35a45f8163be7513dff 4c34bd69-fac8-11f0-a1ba-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=226214 https://iopscience.iop.org/article/10.1088/1402-4896/ada4e7 1 84903cc8-d4cd-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198328 Biotehniška fakulteta Zdravstvena fakulteta 0 0 0