20.500.12556/RUL-166430 Primerjava tradicionalnih in molekularnih metod določanja bentoških nevretenčarjev za namen ocenjevanja kakovosti vodnih ekosistemov Comparison of traditional and molecular methods of benthic invertebrate identification for the purpose of aquatic ecosystems quality assessment Ocenjevanje stanja vodnih ekosistemov je eden izmed ključnih elementov za učinkovito upravljanje in varstvo teh ekosistemov. Pri monitoringu voda s pomočjo bentoških nevretenčarjev, kot kazalcev ekološkega stanja, se tradicionalno še vedno uporablja določanje na podlagi morfoloških značilnosti, ki zahteva veliko znanja in časa. Zato nas je zanimala primerjava z modernimi metodami sekvenciranja in njihova uporabnost v kontekstu monitoringa. Nabrali smo vzorce bentoških nevretenčarjev v litoralu Bohinjskega jezera in primerjali metodo določanja na podlagi morfoloških značilnosti z metabarkodiranjem vzorcev združbe nevretenčarjev in metabarkodiranjem tekočine, v kateri so bili nevretenčarji shranjeni (eDNA – okoljska DNA). Ugotovili smo, da pri računanju indeksa bentoških nevretenčarjev litorala jezer (LBI) z uporabo omenjenih metod pridemo do rezultatov, ki se statistično značilno ne razlikujejo. Z morfološkimi metodami ("morfo") smo zaznali več družin, z analizo DNA združbe nevretenčarjev ("bulk") in DNA shranjevalne tekočine ("eDNA") pa smo dosegli večjo taksonomsko natančnost. Časovno najbolj potratna metoda je bila "morfo", nato "bulk", najhitrejša pa "eDNA". Pregledali smo prednosti in pomanjkljivosti molekularnih metod. Ecological assessment of aquatic ecosystems is one of the key elements for their effective management and conservation. Morphological identification of benthic invertebrates as indicators of ecological status, is still used in monitoring programmes, requiring significant expertise and time. We were interested in comparing these methods with modern sequencing approaches and assessing their applicability in the context of monitoring. We collected samples of benthic invertebrates from the littoral of Lake Bohinj and compared the method of morphological identification with metabarcoding of bulk samples and preservative liquid (eDNA – environmental DNA). Our findings show that the differences between calculated Lake Biotic Indices (LBI) across the three methods are not statistically significant. We detected more invertebrate families using morphological identification but achieved higher taxonomic resolution using bulk or eDNA methods. Morphological identification was the most time consuming, followed by bulk sample metabarcoding, with eDNA metabarcoding being the fastest. Additionally advantages and disadvantages of molecular methods were reviewed. bentoški nevretenčarji določanje morfologija metabarkodiranje sekvenciranje eDNA monitoring primerjava metod Bohinjsko jezero benthic invertebrates identification morphology metabarcoding sequencing eDNA monitoring method comparison lake Bohinj true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-01-12 07:15:07 2025-01-12 07:15:34 2026-01-05 12:51:13 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2027-01-10 255910 45550.pdf 45550.pdf 1 06A0A6ED03D33B097E77F1D8F93961CB 5bf5aca06719cfa1af35f0c477d062a6e16d7ce166fc4f79eb467e1abab74d20 e5cab83f-d0ab-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=198046 Biotehniška fakulteta 0 0 0