20.500.12556/RUL-166368 Defense dynamics in walnut fruit during walnut husk fly (Rhagoletis completa Cresson) infestation an integrative, multi-level analysis : research data underlying the article The walnut husk fly is a critical pest affecting global walnut production, causing economic losses of up to 40-50%. WHF larvae feed within the walnut husk creating necrotic spots, which compromise kernel quality and marketability. In this research, the metabolic defense mechanism of the walnut fruit was targeted including several primary and secondary metabolites (sugars, volatiles, phenolics, fatty acids, carotenoids), enzymes (phenylalanine-ammonia lyase, polyphenol oxidase, peroxidase, glutamine synthetase), and antioxidant activities, using HPLC-MS/MS, GC-MS, and different spectrophotometric methods. Results revealed localized and tissue-specific metabolic responses, with significant changes in volatiles and phenolics, especially naphthoquinones and sesquiterpenes, and enzyme activities. Additionally, prolonged infestations severely affected kernel quality by reducing the content of unsaturated fatty acids and carotenoids, potentially due to oxidative stress and arrested fruit growth. Understanding these defense mechanisms is essential to mitigate economic losses and food waste, ensuring sustainable walnut production through targeted interventions and improved pre- and post-harvest treatments. sugars organic acids fatty acids kernel husk enzymes phenolics volatiles ethylene Rhagoletis completa Juglans regia GC-MS HPLC-MS sladkorji organske kisline maščobne kisline jedrce lupina encimi hlapne snovi etilen true false false Angleški jezik Slovenski jezik Neznano 2025-01-09 07:47:40 2025-01-10 13:24:19 2025-01-11 04:01:40 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno apc 0000-00-00 0000-00-00 0000-00-00 RUL-presentation_and_methodology.pdf RUL-presentation_and_methodology.pdf Materials and methods Materials and methods 1 C079D0D9B6061F7ABA10EDD0D49FAE60 7c2754da3d07199ac00d64846230b6d2ef031c164ec28930466a407417d96889 862884e0-ce55-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=197943 Grohar_-_RUL_original_data.xlsx Grohar_-_RUL_original_data.xlsx 1 8F0742DBA9AF32A5BD108381E359B38A 080de7f6ee533e154f9e6038eca94eccbdf374dc63ad6a8d0f525e7fef470e9f e923260f-ce55-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=197945 Biotehniška fakulteta Poskus Poskus: Teren/Intervencija Meritve in testi: Fizično 0 0 0