20.500.12556/RUL-166010 Assessment of extracellular particles directly in diluted plasma and blood by interferometric light microscopy a study of 613 human and 163 canine samples Extracellular nanoparticles (EPs) are a subject of increasing interest for their biological role as mediators in cell–cell communication; however, their harvesting and assessment from bodily fluids are challenging, as processing can significantly affect samples. With the aim of minimizing processing artifacts, we assessed the number density (n) and hydrodynamic diameter (Dh) of EPs directly in diluted plasma and blood using the following recently developed technique: interferometric light microscopy (ILM). We analyzed 613 blood and plasma samples from human patients with inflammatory bowel disease (IBD), collected in trisodium citrate and ethylenediaminetetraacetic acid (EDTA) anticoagulants, and 163 blood and plasma samples from canine patients with brachycephalic obstructive airway syndrome (BOAS). We found a highly statistically significant correlation between n in the plasma and n in the blood only in the human (i.e., but not canine) blood samples, between the samples with trisodium citrate and EDTA, and between the respective Dh for both species (all p < 10−3). In the human plasma, the average <Dh> was 139 ± 31 nm; in the human blood, <Dh> was 158 ± 11 nm; in the canine plasma, <Dh> was 155 ± 32 nm; and in the canine blood, <Dh> was 171 ± 33 nm. The differences within species were statistically significant (p < 10−2), with sufficient statistical power (P > 0.8). For <n>, we found no statistically significant differences between the human plasma and blood samples or between the samples with trisodium citrate and EDTA. Our results prove that measuring n and Dh of EPs in minimally processed fresh blood and in diluted fresh plasma by means of ILM is feasible for large populations of samples. extracellular particles extracellular vesicles exosomes membrane vesiculation platelets blood products platelet-rich plasma liquid biopsy membrane enclosed drug delivery true false true Angleški jezik Ni določen Članek v reviji 2024-12-17 13:41:18 2024-12-17 13:41:20 2024-12-18 08:57:34 0000-00-00 00:00:00 2024 0 0 21 str. iss. 24, art. 2054 Vol. 13 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 577:576.314 2073-4409 10.3390/cells13242054 219502851 cells-13-02054-v2.pdf cells-13-02054-v2.pdf 1 CCE3E46192DE0F83F0C4BEC5577503AB 5466e9305481cc1e6cc004b8d6e763d04fc3126b119b1d110cd2629981a27110 0c74fb48-bc74-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=197372 https://www.mdpi.com/2073-4409/13/24/2054 1 af47ac4e-bc73-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=197371 Zdravstvena fakulteta Fakulteta za elektrotehniko Fakulteta za farmacijo Medicinska fakulteta Veterinarska fakulteta 0 0 0