20.500.12556/RUL-165961 An experimentally validated numerical model of pH changes in surrogate tissue induced by electroporation pulses Electroporation often leads to electrochemical reactions at the electrode-electrolytic solution interface, particularly when using monophasic pulses of considerable duration (typically on the order of several microseconds or longer) that cause not only capacitive charging of the double-layer, but also faradaic charge transfer between the electrodes and the solution. Applications, where the electrochemical changes are to be either avoided or actively exploited to benefit the treatment, range from gene electrotransfer to electrolytic ablation of tissue. Through numerical modelling and experimental validation, our study explores the extent of pH changes induced by faradaic currents in a surrogate tissue. A mechanistic multiphysics model of pH changes was developed based on first principles, incorporating hydrolysis reactions at the anode and cathode, and the Nernst-Planck model of ion transport. The model was validated using agarose gel tissue phantoms designed to simulate unbuffered and buffered (mimicking in vivo tissue buffering capacity) conditions. An imaging system with pH-sensitive dyes was developed and used to visualise and quantify pH front formation and migration. The model predictions qualitatively aligned well with experimental data, differentiating pH front behaviour between unbuffered and buffered media. However, the quantitative accuracy in predicting the temporal and spatial evolution of the pH fronts can be further improved. Experimental observations emphasise the need for more advanced models. Nevertheless, the developed model provides a sound theoretical foundation for predicting pH changes due to high-voltage electric pulse delivery, such as encountered in electroporation-based treatments and therapies. electrochemistry mathematical model electroporation pH front agarose gel phantoms hydrolysis elektrokemija matematični model elektroporacija pH fronta fantomi iz agaroznega gela true false true Angleški jezik Slovenski jezik Članek v reviji 2024-12-16 09:15:42 2024-12-16 09:15:58 2024-12-17 04:11:06 0000-00-00 00:00:00 2025 0 0 11 str. art. 145363 Vol. 511 20 Jan. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.621:544.6 1873-3859 10.1016/j.electacta.2024.145363 219082243 1-s2.0-S0013468624015998-main.pdf 1-s2.0-S0013468624015998-main.pdf 1 90491CC5AEF38556F60DA673E9297A7D 05d6751b4cd12fedbf1a852935d4891e46312bd3d1c9ce9f22fa704ff48bf946 15f67590-bb86-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=197288 https://www.sciencedirect.com/science/article/pii/S0013468624015998 0 a45ea30e-bb95-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=197293 Fakulteta za elektrotehniko 0 0 0