20.500.12556/RUL-165419 A recommended sampling strategy for genetic identification of Second World War victims in Slovenia Skeletonized human remains from Second World War mass graves in Slovenia are a major challenge in genetic identification, and bones with a high DNA yield must be selected for successful identification. The goal of this study was to construct skeletal sampling strategy recommendations through comparison of the most appropriate groups of skeletal elements. Altogether, 566 bones and teeth from the same mass grave were compared, half analyzed in this study and half in previous studies performed by our group. After anthropological examination, mechanical and chemical cleaning was performed, followed by bone and tooth powdering. Total demineralization of 0.5 g of bone and tooth was followed by extraction and purification of DNA with a Biorobot EZ1 device (Qiagen). The qPCR PowerQuant kit (Promega) was used to measure the amount of DNA, and statistical analysis was performed. Skeletal elements were selected according to known better preservation of DNA in the human body, and they were arranged in seven groups: petrous bone, long bones (femur and tibia), torso bones (first rib and 12th vertebra), metacarpals, metatarsals, short and sesamoid bones (talus, navicular, medial cuneiform, cuboid, calcaneus, and patella), and teeth. Sampling strategy recommendations were constructed based on DNA quantity and quality results. The petrous bone group, metacarpal group, torso bone group, and short and sesamoid bone group produced the highest DNA yields. Accordingly, in addition to standard sampling of long bones (femurs and tibias) and teeth, those additional bone types should be collected for Slovenian Second World War victim identification. genetic identification DNA yield recommendations skeletal sampling Second World War Slovenia genetska identifikacija doprinos DNA priporočila vzorčenje skeleta druga svetovna vojna Slovenija true false true Angleški jezik Slovenski jezik Članek v reviji 2024-12-06 09:54:20 2024-12-06 09:54:23 2024-12-07 04:28:54 0000-00-00 00:00:00 2025 0 0 7 str. art. 112304 Vol. 366 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 61 1872-6283 10.1016/j.forsciint.2024.112304 217791491 1-s2.0-S0379073824003864-main.pdf 1-s2.0-S0379073824003864-main.pdf 1 91750AAEA4D05EEB8792CFB70F89B9A7 9b0da9e7eea0bafaa67554bebd6cdb8aa6dae874a6bf234132a964c9904949d3 0afbd2e2-b3b1-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=196561 https://www.sciencedirect.com/science/article/pii/S0379073824003864 0 3bf81654-b3af-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=196559 Medicinska fakulteta 0 0 0