20.500.12556/RUL-165329 Engineering central metabolism in Yarrowia lipolytica increases lipid accumulation Yarrowia lipolytica, a non-conventional oleaginous yeast, holds great promise for a wide range of metabolic engineering applications. Strains of this species are renowned for their capacity to accommodate high carbon flux through acetyl-CoA and are, therefore, being extensively studied for producing chemicals derived from this precursor molecule. We investigated the effects of the pyruvate bypass, phosphoketolase bypass, and the carnitine shuttle on Y. lipolytica’s central carbon metabolism by monitoring the production of lipids and β-carotene. We constructed a set of parental strains derived from the Y. lipolytica YB-392 wild-type strain, introduced the metabolic bypasses, and subjected the constructed strains to a series of fermentation experiments. We discovered that upon introducing the modifications to central carbon metabolism, carbon is preferentially directed through the lipid biosynthesis pathway, making such engineering an effective strategy for increasing lipid production. The highest lipid content of 0.61 g$_{lipids}$/g$_{DCW}$ was recorded after introducing the carnitine shuttle-associated gene YlCAT2. Meanwhile, the highest increase in production efficiency, 78.2 %, was recorded for a combination of genes associated with the pyruvate bypass (YlPDC1, YlALD3, YlACS1). Yarrowia lipolytica carnitine shuttle phosphoketolase pyruvate bypass lipids β-carotene betakaroten lipidi kvasovke true false true Angleški jezik Slovenski jezik Članek v reviji 2024-12-02 13:13:30 2024-12-02 13:13:33 2024-12-03 04:01:00 0000-00-00 00:00:00 2025 0 0 7 str. Vol. 215, art. 109589 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 57 1873-295X 10.1016/j.bej.2024.109589 217043203 1-s2.0-S1369703X24003760-main.pdf 1-s2.0-S1369703X24003760-main.pdf 1 D1441CC6AA7E7FA81AC354090E750EAA 1c247e2f5d86960939d5335995db69fa74560ecf8bfd8d164903a5c831e21213 c2a99d3c-b0a6-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=196431 https://www.sciencedirect.com/science/article/pii/S1369703X24003760 0 6c616155-b0a6-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=196430 Biotehniška fakulteta 0 0 0