20.500.12556/RUL-165281 Integrative protocol for quantifying cholesterol-related sterols in human serum samples and building decision support systems The growing interest in clinical diagnostics has recently focused on metabolic biomarkers. Here, we present a protocol for sample preparation, extraction of cholesterol-related sterols, and quantification of 10 sterols in human blood serum samples using targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS). We also describe steps of machine learning techniques to develop novel decision-making systems that offer potential benefits in disease monitoring and surveillance by measuring metabolic pathways. For complete details on the use and execution of this protocol, please refer to Kočar et al. (https://doi.org/10.1016/j.isci.2023.107799) and Skubic et al. (https://doi.org/10.3390/molecules25184116). computer sciences metabolism molecular biology računalništvo metabolizem presnova molekularna biologija true false true Angleški jezik Slovenski jezik Članek v reviji 2024-11-29 08:46:31 2024-11-29 08:46:34 2025-11-11 14:52:05 0000-00-00 00:00:00 2024 0 0 23 str. iss. 3, art. 103213 Vol. 5 Jul. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 61:577 2666-1667 10.1016/j.xpro.2024.103213 206706435 RAZ_Kocar_Eva_2024.pdf RAZ_Kocar_Eva_2024.pdf 1 2013F21FE9E5B8B3718F9B27358CE4E5 111d0fd4d7ca8934b2bd7d6589108dfebf11ff11780305a18e7b63a62fa86380 5dbf6861-ae27-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=196359 https://www.sciencedirect.com/science/article/pii/S2666166724003782 0 a3d83e3a-ae25-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=196356 https://star-protocols.cell.com/protocols/3630 0 688bda32-bf02-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=221784 Medicinska fakulteta Fakulteta za računalništvo in informatiko 0 0 0