20.500.12556/RUL-165221 Extracellular vesicles from cerebrospinal fluid revealed changes in miR-19a-3p and miR-4516 expression in Slovene male suicides Suicide is an important public-health concern, with more than 700,000 people dying by suicide yearly. It is a multifactorial phenomenon, shaped by the effects of sociodemographic, environmental and biological factors. The latter two factors can be linked through epigenetic studies, which examine differences in gene expression that are not due to changes in the DNA sequence itself. Epigenetic mechanisms include micro RNAs (miRNAs), which have a direct effect on already translated mRNA, leading to either decay or translational repression of the target mRNA. MiRNA molecules have been identified as cargo of extracellular vesicles (EVs) used by cells for long-distance communication, and pathophysiological changes in miRNA in brain cells may be reflected in cerebrospinal fluid (CSF) vesicles. In this study we investigated the presence and differential expression of selected miRNAs in EVs from the CSF of male suicide completers and controls. Western blot and nanoparticle tracking analyses confirmed the presence of small and medium sized EVs. Of the miRNA analyzed (miR-16-5p, miR-19a-3p, miR-34c-5p, miR-17-5p, miR-4286, miR-26b-5p, miR-381-3p, and miR-4516) miR-19a-3p and miR-4516 reached statistical significance with p-values of 0.0408 and 0.0168, respectively. Mir-4516 and miRNA-19a-3p have been previously studied in suicide, and target SLC6A4 and TNF-α expression, correspondingly. Approximately 70% of known miRNAs are expressed in the central nervous system, and therefore represent an important biomarker potential. Investigating the cargo of CFS and blood EVs would further support the identification of miRNAs with clinical use potential. CSF EVs Slovene male suicides epigenetics miR-19a-3p miR-4516 miRNA expression micro RNA suicidal behavior suicide slovenski samomori moških epigenetika izražanje miRNA mikro RNA samomorilno vedenje samomor true false true Angleški jezik Slovenski jezik Članek v reviji 2024-11-27 10:27:35 2024-11-27 10:27:39 2025-11-14 04:32:59 0000-00-00 00:00:00 2023 0 0 9 str. iss. 6, art. e12868 Vol. 22 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.8:577.2:579 1601-183X 10.1111/gbb.12868 168212483 RAZ_Salamon_Arcan_Iris_2023.pdf RAZ_Salamon_Arcan_Iris_2023.pdf 1 D543EBF2B2EA33F53D62E5723F73950D b0763cfa6d018f2e77679c1e9f637a8c3e5ff6938b93b100a66c9fac9cf6988f 62f7a7fc-c077-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=221847 https://onlinelibrary.wiley.com/doi/10.1111/gbb.12868 1 6f5e7308-aca1-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=196262 Medicinska fakulteta 0 0 0