20.500.12556/RUL-165219 Difference in methylation and expression of brain-derived neurotrophic factor in Alzheimer’s disease and mild cognitive impairment Due to the increasing number of progressive dementias in the population, numerous studies are being conducted that seek to determine risk factors, biomarkers and pathological mechanisms that could help to differentiate between normal symptoms of aging, mild cognitive impairment (MCI) and dementia. The aim of this study was to investigate the possible association of levels of BDNF and COMT gene expression and methylation in peripheral blood cells with the development of Alzheimer’s disease (AD). Our results revealed higher expression levels of BDNF (p < 0.001) in MCI subjects compared to individuals diagnosed with AD. However, no difference in COMT gene expression (p = 0.366) was detected. DNA methylation of the CpG islands and other sequences with potential effects on gene expression regulation revealed just one region (BDNF_9) in the BDNF gene (p = 0.078) with marginally lower levels of methylation in the AD compared to MCI subjects. Here, we show that the level of BDNF expression in the periphery is decreased in subjects with AD compared to individuals with MCI. The combined results from the gene expression analysis and DNA methylation analysis point to the potential of BDNF as a marker that could help distinguish between MCI and AD patients. epigenetics DNA methylation Alzheimer’s dementia gene expression dementia brain-derived neurotrophic factor BDNF catechol-o-methyltransferase COMT cognitive impairment epigenetika metilacija DNA Alzheimerjeva demenca izražanje genov demenca nevrotrofični dejavnik možganskega izvora MDMI katehol-O-metil transferaza COMT kognitivna motnja true false true Angleški jezik Slovenski jezik Članek v reviji 2024-11-27 09:21:23 2024-11-27 09:21:25 2025-11-10 13:46:04 0000-00-00 00:00:00 2023 0 0 17 str. iss. 2, art. 235 Vol. 11 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.8 2227-9059 10.3390/biomedicines11020235 138123267 RAZ_Kouter_Katarina_2023.pdf RAZ_Kouter_Katarina_2023.pdf 1 ADDD54875EDC1BB916A509CB7E9372FE 223fd16f762fae470048da911a672d608034167d642f9225996dad2dd3e62e84 bbd72cfe-ac9a-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=196257 https://www.mdpi.com/2227-9059/11/2/235 1 2ec46a28-ac98-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=196256 Medicinska fakulteta 0 0 0