20.500.12556/RUL-164869 Evaluation of serum diamine oxidase as a diagnostic test for histamine intolerance Histamine intolerance (HIT) is a clinical condition caused by decreased intestinal degradation of ingested histamine, primarily due to reduced enzyme diamine oxidase (DAO) activity, leading to histamine accumulation and causing various clinical manifestations. The measurement of serum DAO is commonly used as the main diagnostic test for HIT, although its diagnostic use is still uncertain. In this retrospective study, we aimed to assess the validity of DAO determination in patients with clinically suspected HIT. We measured DAO levels in 249 patients with suspected HIT and 50 healthy adult controls without HIT-related problems. Based on five clinical criteria, we divided patients into two groups: high (all five inclusion criteria; 41 patients) and low probability of HIT (≤4 inclusion criteria; 208 patients). Patients with a “high probability of HIT” had the lowest DAO (median: 8 U/mL, IQR: 6–10) in comparison to patients with a “low probability of HIT (median: 10 U/mL, IQR: 7–16, p = 0.0006) and healthy controls (median: 18 U/mL, IQR: 14–22, p < 0.0001). The specificity and sensitivity for DAO levels < 3/< 10 U/mL (manufacturer’s set cut-off) to discriminate between patients with “high probability of HIT” and healthy controls were 100%/92% and 2%/71%. On the other hand, the specificity and sensitivity to discriminate between patients with “high probability of HIT” and “low probability of HIT” were 97%/61% and 2%/71%, respectively. Serum DAO determination represents an additional asset to the diagnosis of HIT based on clinical evaluation and assessment, but the diagnosis should not solely rely on DAO measurements. medicine diamine oxidase serum diagnosis histamine intolerance medicina histaminska intoleranca diamin oksidaza serum true false true Angleški jezik Slovenski jezik Članek v reviji 2024-11-14 11:38:43 2024-11-14 11:38:44 2024-11-22 14:56:27 0000-00-00 00:00:00 2023 0 0 9 str. iss. 19, art. 4246 Vol. 15 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 61 2072-6643 10.3390/nu15194246 168484611 nutrients-15-04246.pdf nutrients-15-04246.pdf 1 3C727DED0834F43D5B54C5BD111F865F 20a36109f7de13ee57dfd10b440321419f473d35b4d8a032595ea51a824a84d6 7fa36e54-a274-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=195667 https://www.mdpi.com/2072-6643/15/19/4246 1 411c7572-a274-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=195666 Medicinska fakulteta Biotehniška fakulteta 0 0 0