20.500.12556/RUL-164842 Gene immunotherapy of colon carcinoma with IL-2 and IL-12 using gene electrotransfer Gene immunotherapy has become an important approach in the treatment of cancer. One example is the introduction of genes encoding immunostimulatory cytokines, such as interleukin 2 and interleukin 12, which stimulate immune cells in tumours. The aim of our study was to determine the effects of gene electrotransfer of plasmids encoding interleukin 2 and interleukin 12 individually and in combination in the CT26 murine colon carcinoma cell line in mice. In the in vitro experiment, the pulse protocol that resulted in the highest expression of IL-2 and IL-12 mRNA and proteins was used for the in vivo part. In vivo, tumour growth delay and also complete response were observed in the group treated with the plasmid combination. Compared to the control group, the highest levels of various immunostimulatory cytokines and increased immune infiltration were observed in the combination group. Long-term anti-tumour immunity was observed in the combination group after tumour re-challenge. In conclusion, our combination therapy efficiently eradicated CT26 colon carcinoma in mice and also generated strong anti-tumour immune memory. colon carcinoma gene electrotransfer gene immunotherapy CT26 colon carcinoma IL-2 IL-12 anti-tumour immune memory rak debelega črevesa elektroprenos genov genska imunoterapija true false true Angleški jezik Slovenski jezik Članek v reviji 2024-11-13 14:12:28 2024-11-13 14:12:32 2024-11-22 14:56:21 0000-00-00 00:00:00 2023 0 0 25 str. iss. 16, art. 12900 Vol. 24 2023 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 602.6/.7 1422-0067 10.3390/ijms241612900 162334211 ijms-24-12900.pdf ijms-24-12900.pdf 1 DA9CE0EFC9195E902CCA271685FE608E e3062bd270d2225166fdb1e5bdf9e71dd40e183b00749eca03bc7d94728d204c f4985d6c-a1c0-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=195609 https://www.mdpi.com/1422-0067/24/16/12900 1 94afce74-a1c0-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=195608 Medicinska fakulteta Zdravstvena fakulteta 0 0 0