20.500.12556/RUL-164649 Comprehensive analysis of 66 complete molluscum contagiosum virus (MOCV) genomes characterization and functional annotation of 47 novel complete MOCV genomes, including the first genome of MOCV genotype 3, and a proposal for harmonized MOCV genotyping indexing Four molluscum contagiosum virus (MOCV) genotypes (MOCV1–4) and four subtype variants (MOCV1p, MOCV1va, MOCV1vb, and MOCV1vc) were partially characterized using restriction enzyme profiling in the early 1980s/1990s. However, complete genome sequences of only MOCV1 and MOCV2 are available. The evolutionary pathways of MOCV genotypes and subtype variants with unavailable sequences remain unclear, and also whether all MOCV genotypes/subtype variants can be reliably detected and appropriately categorized using available PCR-based protocols. We de novo fully characterized and functionally annotated 47 complete MOCV genomes, including two putative non-MOCV1/2 isolates, expanding the number of fully characterized MOCV genomes to 66. To ascertain the placement of any putative novel MOCV sequence into the restriction profiling typing scheme, we developed an original framework for extracting complete MOCV genome sequence-based restriction profiles and matching them with reference restriction profiles. We confirmed that two putative non-MOCV1/2 isolates represent the first complete genomes of MOCV3. Comprehensive phylogenomic, recombination, and restriction enzyme recognition site analysis of all 66 currently available MOCV genomes showed that they can be agglomerated into six phylogenetic subgroups (PG1–6), corresponding to the subtype variants from the pioneering studies. PG5 was a novel subtype variant of MOCV2, but no PGs corresponded to the subtype variants MOCV1vb or MOCV4. We showed that the phylogenetic subgroups may have diverged from the prototype MOCV genotype lineages following large-scale recombination events and hinted at partial sequence content of MOCV4 and direction of recombinant transfer in the events that spawned PG5 and the yet undetected subtype variant MOCV1vb. complete genome restriction mapping complete genome sequencing de novo assembly genome annotation genomic diversity molluscipoxvirus harmonization molluscum contagiosum virus molluscum contagiosum virus genotyping recombination popolno kartiranje omejitve genoma popolno sekvenciranje genoma anotacija genoma genomska raznolikost harmonizacija moluscipoksvirusa virus nalezljivega moluskuma genotipizacija virusa nalezljivega moluskuma rekombinacija true false true Angleški jezik Slovenski jezik Članek v reviji 2024-11-06 11:30:30 2024-11-06 11:30:35 2024-11-22 14:55:13 0000-00-00 00:00:00 2023 0 0 18 str. iss. 6, art. e02224-23 Vol. 14 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-097:579 2150-7511 10.1128/mbio.02224-23 172346371 zorec-et-al-2023-comprehensive-analysis-of-66-complete-molluscum-contagiosum-virus-(mocv)-genomes-characterization-and.pdf zorec-et-al-2023-comprehensive-analysis-of-66-complete-molluscum-contagiosum-virus-(mocv)-genomes-characterization-and.pdf 1 9ABD606E940A50D00E9667A164999B44 0f99a4fa184623c0b13c6503850e5fdc55b7e22afa8255e4dc07651769aedc3b 2e73a62e-9c2a-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=195239 https://journals.asm.org/doi/10.1128/mbio.02224-23 0 0b6f0faa-9c2a-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=195238 Medicinska fakulteta 0 0 0