20.500.12556/RUL-164589 Can polymeric nanofibers effectively preserve and deliver live therapeutic bacteria? Probiotics and live therapeutic bacteria (LTB), their strictly regulated therapeutic counterpart, are increasingly important in treating and preventing biofilm-related diseases. This necessitates new approaches to (i) preserve bacterial viability during manufacturing and storage and (ii) incorporate LTB into delivery systems for enhanced therapeutic efficacy. This review explores advances in probiotic and LTB product development, focusing on preservation, protection, and improved delivery. Preservation of bacteria can be achieved by drying methods that decelerate metabolism but introduce stresses affecting viability which can be mitigated with suitable excipients like polymeric or low molecular weight stabilizers. The review emphasizes the incorporation of LTB into polymer-based nanofibers via electrospinning, enabling simultaneous drying, encapsulation, and delivery system production. Optimization of bacterial survival during electrospinning and storage is discussed, as well as controlled LTB release achievable through formulation design using gel-forming, gastroprotective, mucoadhesive, and pH-responsive polymers. Evaluation of the presence of the actual therapeutic strains, bacterial viability and activity by CFU enumeration or alternative analytical techniques is presented as a key aspect of developing effective and safe formulations with LTB. This review offers insights into designing delivery systems, especially polymeric nanofibers, for preservation and delivery of LTB, guiding readers in developing innovative biotherapeutic delivery systems. probiotics electrospinning excipients drying viability biotherapeutics elektropredenje pomožne snovi sposobnost preživetja bioterapevtiki probiotiki sušenje terapevtska uporaba true false true Angleški jezik Slovenski jezik Članek v reviji 2024-11-04 13:52:11 2024-11-04 13:52:19 2024-11-22 14:54:57 0000-00-00 00:00:00 2025 0 0 17 str. art. 114329 Vol. 245 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 613.292:615 0927-7765 10.1016/j.colsurfb.2024.114329 212531971 1-s2.0-S0927776524005885-main.pdf 1-s2.0-S0927776524005885-main.pdf 1 3F458BA08F24463CE38F5F865F2DA70B b7cc0ffb5201560d4942758461221bdae98e4e880a41a42967870fe49f1687f8 90b9772e-9aab-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=195122 https://www.sciencedirect.com/science/article/pii/S0927776524005885 1 4ba86ab0-9aab-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=195121 Fakulteta za farmacijo 0 0 0