20.500.12556/RUL-164495 Inactivation of MS-2 virus in water by rotational generator of hydraulic shock This study investigates the effect of hydraulic shock waves on inactivation of MS-2 bacteriophage, a norovirus surrogate. A falling circular jet of water spiked with the MS-2 (∼1000 PFU/mL) was repeatedly impacted by a rotating blade, resulting in occurrence of hydraulic shock waves within the liquid region adjacent to the impact. The proof-of-concept rotational generator of hydraulic shock treating 9 L of water spiked with viruses was able to achieve 3 logs reduction of viral plaque count within 80–100 liquid passes at moderate blade impact velocities (namely, 70 and 88 m/s) despite the water temperature not exceeding 40 °C and no detectible cavitation. Within the first 20 liquid passes, most MS-2 capsid proteins were degraded, with their concentration reduced from 22 μg/mL to only 7.3 μg/mL. Due to the lack of further capsid protein destruction, additional reduction in MS-2 plaque count in subsequent 80 passes is indicative of damage inflicted to the viral recognition receptors. All this suggests that shockwaves of moderate amplitude (few tens of MPa) alone are sufficient for effective viral inactivation. Considering this and the device's good scalability potential, rotational hydraulic shock generators could prove effective in treating virus-contaminated waters. viral inactivation MS-2 bacteriophage capsid protein hydraulic shock shockwaves hydrodynamic cavitation inaktivacija virusov kapsida hidravlični udar kavitacija true false true Angleški jezik Slovenski jezik Članek v reviji 2024-10-28 07:13:15 2024-10-28 07:13:23 2025-02-06 14:09:09 0000-00-00 00:00:00 2024 0 0 11 str. iss. 21, [article no.] e34037 Vol. 10 Nov. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 532.528 2405-8440 10.1016/j.heliyon.2024.e39795 213069059 Bizjan_..._Inactivation_of_MS-2_virus_in_water_by_rotational_generator_....pdf Bizjan_..._Inactivation_of_MS-2_virus_in_water_by_rotational_generator_....pdf 1 7EEDE751175F751DCE33F5DCD6EE2499 eba112b9c903ede85e8217f88269279d0429f1c5a9f9162aa3b35b48b5c3cc56 8a363eb9-94f4-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=194969 https://www.sciencedirect.com/science/article/pii/S2405844024158264 1 6cc2a82f-94f3-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=194968 Fakulteta za strojništvo Fakulteta za gradbeništvo in geodezijo 0 0 0