20.500.12556/RUL-164423 Viskoelastične lastnosti adsorbiranih vmesnih plasti in agregacija proteinov zaradi izpostavljenosti mehanskemu stresu Viscoelastic properties of adsorbed interfacial layers and mechanical stress induced protein aggregation Proteinske raztopine se pogosto uporabljajo v farmacevtski industriji, a je za njihovo uporabo pomembno, da so stabilne in da se proteini ne združujejo v večje agregate. V magistrskem delu smo obravnavali lastnosti adsorbiranih molekulskih plasti, ki nastanejo na fazni meji tekočina-plin v raztopinah proteinov, oz. konkretno v našem primeru bioloških zdravilih. Za določanje viskoelastičnih lastnosti vzorcev smo uporabili površinski reometer, ki meri odziv s pomočjo namagnetene igle, ki jo vzbujamo z oscilirajočim magnetnim poljem dveh tuljav. Iste proteinske vzorce smo izpostavili nadzorovanemu mehanskemu stresanju ter s pomočjo pretočne mikroskopije analizirali nastale proteinske mikrodelce. Poskusili smo ugotoviti, ali obstaja korelacija med viskoelastičnimi lastnostmi površinske adsorbirane plasti in distribucijo zaradi mehanskega stresa induciranih proteinskih mikrodelcev. Protein solutions are often used in the pharmaceutical industry, but it is important for their use to be stable and for the proteins to not aggregate into larger particles. In this master thesis, we have studied the properties of adsorbed molecular layers formed at the liquid-gas interface in protein solutions, or more specifically in our case, in biopharmaceuticals. To determine the viscoelastic properties, we used a surface rheometer that measures the response using a magnetized needle excited by an oscillating magnetic field of two coils. The same protein samples were subjected to controlled mechanical shaking and the resulting protein microparticles were analysed by flow imaging microscopy. We sought to determine whether there is a correlation between the viscoelastic properties of the surface adsorbed layer and the distribution of the mechanical stress-induced protein microparticles. proteini monoklonska protitelesa površinski reometer površinska viskoelastičnost pretočna mikroskopija proteinski agregati proteins monoclonal antibodies surface rheometer surface viscoelasticity flow imaging microscopy protein aggregates true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-10-25 08:15:05 2024-10-25 08:15:11 2024-11-22 14:54:16 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 145408 213235715 15494.pdf 15494.pdf 1 33889C4938A984FD2070583BFBD04B79 df887b1ace192b268c06e57f164bffdb3232eedb1e077be2cf77d914845669b2 31505037-9298-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=194790 Fakulteta za matematiko in fiziko 0 0 0