20.500.12556/RUL-164295 Analiza proteoma hemolimfe raka enakonožca Porcellio scaber po izpostavitvi mikroplastiki Proteome analysis of isopod Porcellio scaber haemolymph after microplastics exposure Plastika je danes velik okoljski problem, saj njeni odpadki trajno onesnažujejo okolje. Poseben problem predstavlja mikroplastika (MP), majhni delci plastike (< 1 mm), ki nastajajo ob razgradnji večjih kosov in so biološko bolj dostopni. MP je prisotna v kopenskih in vodnih okoljih ter vpliva na organizme, vključno s kopenskimi raki enakonožci vrste Porcellio scaber, ki so bili predmet študije. Namen magistrskega dela je bil z uporabo proteomike preučiti morebitne spremembe v hemolimfi ob izpostavljenosti modelnega organizma MP. Opazovali smo učinek različnih vrst in koncentracij MP na izražanje proteinov v hemolimfi rakov P. scaber. Ugotovili smo, da izpostavljenost MP povzroča spremembe v izražanju proteinov, pri čemer so višje koncentracije MP povzročile izrazitejše spremembe. Raziskava je pokazala, da konvencionalna in biorazgradljiva mikroplastika obe povzročita spremembe v proteomu hemolimfe, kar zavrača hipotezo, da bi biorazgradljiva mikroplastika imela manjši vpliv. Ugotovljene spremembe v izražanju proteinov kažejo na stresni odziv organizmov na prisotnost MP v okolju, vključno z aktivacijo imunskega sistema in spremembami v metabolizmu. Študija potrjuje, da je MP resen okoljski dejavnik, ki vpliva na fiziološke procese pri organizmih, ter izpostavlja potrebo po nadaljnjih raziskavah njenega vpliva. Plastics, which were developed in the 19th century, have become a major environmental problem today, as their waste permanently pollutes the environment. One particular problem is microplastics (MP), small plastic particles that are formed when larger parts decompose and are more biologically accessible. MP occurs in both terrestrial and aquatic environments and affects organisms, including terrestrial isopods of the species Porcellio scaber, which were the subject of this study. The aim of the MSc thesis was to use proteomics to investigate potential changes in the hemolymph when the model organism is exposed to MP. The response to different types and concentrations of MP on protein expression in the hemolymph of the isopod P. scaber was observed. It was found that exposure to MP leads to changes in protein expression, with higher concentrations of MP leading to more pronounced changes. The study showed that conventional and biodegradable plastics cause similar changes in the hemolymph proteome, disproving the hypothesis that biodegradable plastics would have a lesser effect. The observed changes in protein expression indicate a stress response of the organisms to the presence of MP, including activation of the immune system and changes in metabolism. The study confirms that MP is a serious environmental factor affecting physiological processes in organisms and emphasises the need for further research of its effects. mikroplastika proteom LDPE PBAT proteini mikroplastic proteom LDPE PBAT protein true false false [K. Kukenberger] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-10-20 07:15:10 2024-10-20 07:15:23 2024-10-25 10:39:11 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 591.113(043.2) 248378 212947203 44165.pdf 44165.pdf 1 881A633D887860DAB9D8DA4E34AEBAFF c346ce8fbd642f6a203de4b82f98d5c340d9b63a8e6d5d91e7c329983c8ed00e 0e1304e4-8ea2-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=194596 44166.pdf 44166.pdf 1 3CA7283679EA8098D20467305AEE0790 c3fb94ed5adb9117464cb7e964d8a4d45ee70ee9454ed870a290124f7f28584e 0e25a10d-8ea2-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=194597 Biotehniška fakulteta 0 0 0