20.500.12556/RUL-164267 Navigational signals for insect and slug parasitic nematodes the role of ascorbate–glutathione system and volatiles released by insect-damaged sweet pepper roots This study of underground multitrophic communication, involving plant roots, insects, and parasitic nematodes, is an emerging field with significant implications for understanding plant–insect–nematode interactions. Our research investigated the impact of wireworm (Agriotes lineatus L. [Coleoptera: Elateridae]) infestations on the ascorbate–glutathione system in sweet pepper (Capsicum annuum L.) plants in order to study the potential role in root-exudate-mediated nematode chemotaxis. We observed that an A. lineatus infestation led to a decrease in leaf ascorbate levels and an increase in root ascorbate, with corresponding increases in the glutathione content in both roots and leaves. Additionally, a pigment analysis revealed increased carotenoid and chlorophyll levels and a shift towards a de-epoxidized state in the xanthophyll cycle. These changes suggest an individual and integrated regulatory function of photosynthetic pigments accompanied with redox modifications of the ascorbate–glutathione system that enhance plant defense. We also noted changes in the root volatile organic compound (VOC). Limonene, methyl salicylate, and benzyl salicylate decreased, whereas hexanal, neoisopulegol, nonanal, phenylethyl alcohol, m-di-tert-butylbenzene, and trans-β-ionone increased in the roots of attacked plants compared to the control group. Most notably, the VOC hexanal and amino acid exudate cysteine were tested for the chemotaxis assay. Nematode responses to chemoattractants were found to be species-specific, influenced by environmental conditions such as temperature. This study highlights the complexity of nematode chemotaxis and suggests that VOC-based biological control strategies must consider nematode foraging strategies and environmental factors. Future research should further explore these dynamics to optimize nematode management in agricultural systems. Capsicum annuum Agriotes lineatus ascorbate–glutathione system root volatile compounds entomopathogenic nematodes slug parasitic nematodes entomopatogene ogorčice obrambni mehanizem rastlin varstvo rastlin true false true Angleški jezik Slovenski jezik Članek v reviji 2024-10-18 10:47:36 2024-10-18 10:47:44 2024-10-19 04:25:47 0000-00-00 00:00:00 2024 0 0 14 str. no. 10, art. 805 Vol. 15 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 632 2075-4450 10.3390/insects15100805 212072195 Navigational_Signals_for_Insect_and_Slug_Parasitic_Nematodes__The_Role_of_Ascorbate–Glutathione_System_and_Volatiles_Released_by_Insect-Damaged_Sweet_Pepper_Roots.pdf Navigational_Signals_for_Insect_and_Slug_Parasitic_Nematodes__The_Role_of_Ascorbate–Glutathione_System_and_Volatiles_Released_by_Insect-Damaged_Sweet_Pepper_Roots.pdf 1 906CD2F415F4D66CE8E5A99384814E2A dc1c2a71c1d258fa42a6b2e50722272b2d644e5bb8ee091fc50647d9ecd8f7c0 c1c380d2-8d2d-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=194530 https://www.mdpi.com/2075-4450/15/10/805 1 63c34176-8d2d-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=194528 Biotehniška fakulteta 0 0 0