20.500.12556/RUL-163839 Analiza izražanja z metilacijo DNA povezanih genov hmelja ob okužbi s CBCVd Analysis of the expression of DNA-methylation-related genes in hops (Humulus lupulus) upon CBCVd infection V magistrskem delu smo s qPCR preučevali izražanje genov, povezanih z metilacijo DNA, pri hmelju, okuženem z viroidom CBCVd. Hmelj je pomembna kmetijska rastlina, ki jo ogrožajo različni patogeni, med katerimi je okužba s CBCVd še posebej problematična. Osredotočili smo se na gene DRM2, AGO4, CLSY1, RDR2, HEN1 in DCL3, ki so povezani z metilacijo DNA, epigenetskim mehanizmom, s katerimi se rastline hitro prilagajajo na biotske in abiotske dražljaje. Eksperiment smo izvedli na treh sortah hmelja, v treh časovnih točkah po okužbi. Naša hipoteza je bila, da bo izražanje genov različno med okuženimi in neokuženimi rastlinami, pričakovali pa smo povišano izražanje pri okuženih rastlinah, kar bi nakazovalo aktivno metilacijo kot obrambni odziv rastline na okužbo. Rezultati so pokazali statistično značilne razlike v izražanju genov AGO4, HEN1 in DCL3, vendar le v nekaterih časovnih točkah. Presenetljivo je bilo, da je bilo izražanje teh genov višje pri neokuženih rastlinah. Pri sorti Celeia, ki je občutljiva na okužbo, smo pričakovali višje izražanje genov v primerjavi s tolerantnima sortama Styrian Styrian Cardinal in Styrian Styrian Wolf. Statistično značilna razlika v izražanju je bila opažena le pri genu AGO4, kjer je bilo izražanje višje pri domnevno tolerantni sorti Styrian Styrian Wolf, kar nakazuje na kompleksnost odziva rastlin na viroidne okužbe in potrebo po nadaljnjih raziskavah na tem področju. In this master's thesis, we used qPCR to study the expression of genes related to DNA methylation in hop plants infected with the CBCVd viroid. Hops are an important agricultural crop threatened by various pathogens, with CBCVd infection being particularly problematic. We focused on the genes DRM2, AGO4, CLSY1, RDR2, HEN1, and DCL3, which are associated with DNA methylation, an epigenetic mechanism by which plants rapidly adapt to biotic and abiotic stimuli. The experiment was conducted on three hop varieties at three different time points post-infection. Our hypothesis was that gene expression would differ between infected and uninfected plants, with higher expression expected in infected plants, indicating active methylation as a defense response to the infection. The results showed statistically significant differences in the expression of the AGO4, HEN1, and DCL3 genes, but only at certain time points. Surprisingly, the expression of these genes was higher in uninfected plants. For the variety Celeia, which is sensitive to infection, we expected higher gene expression compared to the tolerant varieties Styrian Styrian Cardinal and Styrian Styrian Wolf. However, a statistically significant difference in expression was observed only for the AGO4 gene, where expression was higher in the reportedly tolerant Styrian Styrian Wolf variety, highlighting the complexity of plant responses to viroid infections and the need for further research in this area. viroidi CBCVd transkripcijsko utišanje genov metilacija viroids CBCVd transcriptional gene silencing methylation true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-10-12 07:15:14 2024-10-12 07:15:56 2024-10-14 10:01:04 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 248289 211352835 45085.pdf 45085.pdf 1 D2CEFF2F13EAC4D153EB0D54F54061FB f6e223562626b3eb76c9346541cf7a3a3b7ab5c13434fdaf823da9988081e7c1 e04cd07a-8858-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=193788 Biotehniška fakulteta 0 0 0