20.500.12556/RUL-163678 Membransko aktivni proteini v mahovih Membrane-active proteins in bryophytes Mahovi (Bryophyta) tvorijo drugo največjo taksonomsko skupino v rastlinskem kraljestvu in imajo pomembno ekološko vlogo. Hkrati so mahovi potencialni vir biološko aktivnih spojin za uporabo v farmaciji in biotehnologiji. Kljub temu potencialu ostajajo spregledana skupina organizmov. Do danes je še vedno malo znanega o njihovih membransko aktivnih proteinih. V raziskavi smo zato preučevali vodne izvlečke mahov, z namenom, da bi ugotovili ali ti izvlečki vsebujejo potencialno membransko-aktivne proteine, ki bi bili litični za celični model sesalcev (goveji eritrociti) in/ali žuželk (žuželčja celična linija Sf9). Nadalje smo želeli raziskati, ali se ti proteini vežejo na membranske lipide, kar bi lahko pomenilo, da lahko litično aktivnost vodnih izvlečkov mahov pripišemo delovanju membransko aktivnih proteinov na lipidne receptorje. V raziskavi smo ovrednotili kuhane in surove vodne izvlečke izbranih vrst mahov, ki uspevajo v Sloveniji in ugotovili, da mahovi vsebujejo proteine, ki prepoznajo in se vežejo na ustrezne lipidne receptorje. Slednje smo zaznali pri 10 % oz. štirih vrstah analiziranih vrst mahov. Nekateri od izvlečkov, ki so vsebovali proteine, ki prepoznajo lipidne receptorje so delovali hkrati tudi citolitično. Taka sta bila vodna izvlečka iz vrst Lunuaria cruciata in Marchantia quadrata, katerima smo prvi pripisali citolitični potencial, ki bi lahko bil posledica vsebnosti membransko aktivnih proteinov. Mosses (Bryophyta) form the second largest taxonomic group in the plant kingdom and play an important ecological role. At the same time, mosses are a potential source of biologically active compounds for pharmaceutical and biotechnological applications. Despite this potential, they remain an overlooked group of organisms. To date, little is known about their membrane active proteins. Therefore, in this study we investigated aqueous extracts of mosses to determine whether these extracts contain potential membrane-active proteins that would be lytic for a mammalian (bovine erythrocytes) and/or an insect (Sf9 insect cell line) cell model. We also aimed to determine whether these proteins at the same time bind to membrane lipids, which could imply that the lytic activity of aqueous extracts of mosses could be attributed to the action of membrane-active proteins on lipid receptors. In this study, we evaluated raw aqueous extracts (with or without the heat-treatment) of selected moss species naturally thriving in Slovenia and found that some of these mosses contain proteins that recognize and bind to the membrane lipid receptors. The latter was detected in 10 % (e.g. in four species) of the mosses analysed. Some of the lipid receptor-binding proteins were cytolytic. This was the case for the aqueous extracts of Lunuaria cruciata and Marchantia quadrata, to which we first attributed a cytolytic potential, which could be due to the content of membrane-active proteins. mahovi proteini membransko aktivni proteini membrane citotoksičnost hemolitičnost mosses proteins membrane-active proteins membranes cytotoxicity haemolytic activity true false false [N. Paternost] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-10-10 08:16:15 2024-10-10 08:16:19 2024-10-17 12:36:18 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 582.32:576.314(043.2) 248280 211948291 45052.pdf 45052.pdf 1 8FE8F037C67AD7C8D8FCDC1777DFA3D4 eb692033ceaf4db68e85dba35b5dbe3022621fec89d88d9e56853850e5d83fea fce44707-86ce-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=193554 Biotehniška fakulteta 0 0 0