20.500.12556/RUL-163668 Time scale analysis of enzymatic reduction of uric acid in a microfluidic biomedical device Time Scale Analysis (TSA) is an investigative tool used in engineering design to identify locations in processes that should be a focus of Process Intensification (PI). Furthermore, TSA points to process variables and parameters that could be used to advance and measure PI improvement. However, TSA cannot suggest any specific design solution to intensify process performance. Instead, design engineers should use their fundamental knowledge and creative intelligence to specify detailed design transformations. TSA will then provide a specific quantitative measure of the improvement. TSA implementation improves an explicitly defined process performance, thus helping achieve process intensification goals. TSA is based on first principles, and it utilizes Characteristic Times (CT) such as diffusion, mean residence, and reaction times to improve an existing process. In this study, we specifically consider microfluidic biomedical devices. To illustrate the genesis of CT and TSA, we start by developing a mathematical model of an enzymatic degradation process in a biomedical device called iCore based on mass, momentum, and kinetic equations. After introducing user-defined scaling parameters, we extract CTs pertinent to the enzymatic degradation of uric acid in this microfluidic biomedical device. Diffusion coefficients, microchannel architectural characteristics, enzyme loading, hydrogel thickness, and characteristic parameters of enzyme kinetics are the parameters and process variables incorporated in this analysis. Finally, we compared the extracted CTs with a COMSOL Multiphysics parametric study to demonstrate how time scale analysis as a design tool and adjusting design parameters, such as diffusion coefficient, hydrogel layer thickness, substrate concentration, and enzyme concentration, can enhance the enzymatic reaction process without a need for complex computational modeling. It is crucial to recognize that pertinent CTs can be determined by understanding the type and nature of the observed process, previous experience, published data, and other foundational engineering design work. There is no need for mathematical modeling and numerical simulations to identify and acknowledge the CTs relevant and essential to the observed process; in this work, we only illustrate the principal origin of CTs via a detailed mathematical model of the process, as previously reported by Jovanovic et al. Therefore, in a routine application of TSA, it is important to remember that mathematical modeling and detailed numerical simulations are not necessary. This is a very comforting fact when TSA is deployed as a tool in higher-level process design functions. The investigations on how best to apply TSA in these higher level design functions such as Process Intensification, scale-up/numbering-up, change of device architecture, change of operating conditions, change of process feed characteristics, change of material physical and chemical properties, parametric optimization of the system for various objective functions, and techno-economic analysis, are yet to be studied and reported. time scale analysis process intensification characteristic times microfluidics uric acid enzymatic reduction analiza časovnih nivojev procesna intenzifikacija karakteristični časi mikrofluidika encimska redukcija sečne kisline true false true Angleški jezik Slovenski jezik Članek v reviji 2024-10-10 07:51:27 2024-10-10 07:51:30 2024-10-11 04:24:10 0000-00-00 00:00:00 2024 0 0 9 str. Vol. 6, art. 1451222 8 Oct. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.02.098 2673-2718 10.3389/fceng.2024.1451222 210778115 RAZ_Asgharpour_Masouleh_Alireza_2024.pdf RAZ_Asgharpour_Masouleh_Alireza_2024.pdf 1 FA0D66C4812C6FD2638930C66E8C7EF0 d83e1e3961238cce0f56bd380c8396bb934e6b8d3f75540d0ce808177b7bcc29 78d0afd4-86cc-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=193542 https://www.frontiersin.org/journals/chemical-engineering/articles/10.3389/fceng.2024.1451222/full 1 853f1340-86cb-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=193541 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0