20.500.12556/RUL-162303 Hidrogeli na osnovi ciklodekstrina in izbranih polisaharidov Hydrogels based on cyclodextrin and selected polysaccharides Napredni nosilni materiali za mikrokapsulacijo so manj dostopni za uporabo v živilski industriji. V delu smo se osredotočili na nove nosilce na osnovi beta ciklodekstrina in alginata. Uporabili smo nativni ciklodekstrin, ki smo ga funkcionalizirali s citronsko kislino in maleinsko kislino. Ciklodekstrin citrat smo okarakterizirali s pomočjo spektrometrskih ter morfoloških tehnik, medtem ko smo ciklodekstrin maleat okarakterizirali s spektroskopijsko metodo NMR. Preizkusili smo kombinacijo ciklodekstrinov, alginata ter zamreževalnih sredstev za oblikovanje hidrogelov. Preverili smo kako vrsta in koncentracija gradnikov vplivata na oblikovanje in na časovno dinamiko želiranja hidrogelov. S FTIR spektroskopijo smo potrdili nastanek estrske vezi med ciklodekstrinom in citronsko kislino. Z metodama NMR in MS smo opredelili molekuli ciklodekstrin citrata (stopnja funkcionalizacije 1) in maleata (stopnja funkcionalizacije 3). Med procesom želiranja smo opazili, da vključki ciklodekstrin citrata in maleata v alginatni gel upočasnjujejo želiranje. Prisotnost ciklodekstrinov v hidrogelih alginata zmanjša območje linearnosti viskoelastičnosti. Z UV-vis spektrometrijo smo ugotovili podoben potek in obseg sproščanja vseh treh oblik ciklodekstrinov iz mikrokroglic (od 12 do 17 % v 28 urah). Nadaljnje raziskave hidrogelov s še nižjimi koncentracijami ciklodekstrinov, bi na podlagi naših raziskav, podale še podrobnejše informacije o strukturnih lastnostih na novo pridobljenih materialov. Advanced carrier materials for microencapsulation are rarely available for use in the food industry. In this thesis, we focused on developing new carrier materials based on beta-cyclodextrin and alginate. We used native cyclodextrin, on which we performed functionalization with citric and maleic acid. Cyclodextrin citrate was characterised via spectrometric and morphological techniques, while cyclodextrin maleate was characterized by NMR spectroscopy. We tested combinations of cyclodextrins, alginate and crosslinking agents for hydrogel formation. We examined how types and concentrations of components affected gel formation and time-dependant gelation behaviour. FTIR spectroscopy confirmed newly formed ester bond between beta-cyclodextrin and citric acid. NMR spectroscopy and mass spectrometry identified molecules of cyclodextrin citrate (degree of substitution 1) and maleate (degree of substitution 3). During gelation, the cyclodextrin citrate and maleate slowed down the alginate gelation process. Presence of cyclodextrin compounds in alginate hydrogel reduces the range of linear viscoelasticity. UV-vis spectrometry indicated a similar release pattern and release range of all three forms of cyclodextrins from microspheres (from 12 to 17 % in 28 hours). Based on our research, it would be also prudent to further study lower cyclodextrin concentrations, to gain additional information on structure and gelation characteristics of the newly obtained materials. hidrogeli alginat ciklodekstrini beta-ciklodekstrin beta-ciklodekstrin citrat beta-ciklodekstrin maleat viskoelastične meritve želiranje mikrokapsulacija hydrogels alginate cyclodextrins beta-cyclodextrin beta-cyclodextrin citrate beta-cyclodextrin maleate viscoelasticity measurement gelation encapsulation true false false [A. Damjanović] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-09-21 07:16:39 2024-09-21 07:16:45 2024-09-24 03:32:59 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 664.0:544.773.432:615.014.6 246056 208437763 43235.pdf 43235.pdf 1 4A62C3F33CAC4A734C6108F5A1E40976 7cf040650d090b4110cc14d36ee3bd540ddd102937ad1624e363e2da50bf0749 957da386-77d8-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=191509 Biotehniška fakulteta 0 0 0